FastQCFastQC Report
Tue 8 Aug 2023
SRR2518206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2518206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65951935
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA32489474.926234537318731No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT26435544.0083039261850315No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA25477753.8630784676749816No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA17477102.649975319147194No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC11671831.7697479232413726No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA9385741.423118214802947No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA8081931.2254272751815394No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA7940841.2040344229475601No Hit
AGAGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA7279281.1037250082200014No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA6923081.0497159787654449No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT6740761.0220716041159368No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT6341120.9614759597273377No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG5711200.8659639781607621No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT3648540.5532119717184947No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC3278590.49711809062160195No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG3052820.46288558478231157No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA2983860.4524294852000931No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCTGTAGGCACCATCAA2946770.44680569266087494No Hit
TAGAGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA2686600.40735726707639436No Hit
CGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATCA2597130.39379132697168023No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA2453670.37203912212734924No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA2353160.3567992356858066No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA2341360.3550100539127472No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2233970.33872698352216657No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA2173690.3295869939221647No Hit
ACGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT2010410.30482957020138984No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1533070.23245261871391643No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA1522470.2308453876296427No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1519060.23032834442234335No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCCTGTAGGCACCATC1494820.2266529405088721No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATAG1494760.22664384297443282No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT1468170.2226121189620896No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1461880.22165839410170454No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA1460680.2214764434129188No Hit
AGCGGGCCCCCGGTGGGGAGGGGGGCCCGGACACCTGTAGGCACCATCAA1441940.21863498015638208No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1406880.21331898753235973No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAATA1294500.19627930552757852No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT1189440.18034952272439012No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGGCACCATCAAT1187940.18012208436340799No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGAACCATCAAT1149780.1743360524600226No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA1098240.16652127037667658No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGAACCATCA1040570.15777702352478362No Hit
CAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA1030850.1563032229456194No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA993820.15068852794084056No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA983160.14907219932212754No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACAATCAAT876710.13293165697109569No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA868650.13170955484475172No Hit
AGAGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA838080.12707436104793587No Hit
ACTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC797390.12090471644236064No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT779050.11812390341541912No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC770550.11683508603652039No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAATA768720.11655761123612218No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATCG732620.11108392801515224No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCTGTAGGCACCATCAAT695710.10548742807925196No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCCTGTAGGCACCAT695520.10545861922019423No Hit
CGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAATA685140.10388474576219787No Hit
TCGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATC682780.10352690940758598No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGAACCATCAA682150.1034313852959735No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA674140.10221686444832892No Hit
TAGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT661590.10031396349477843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)