FastQCFastQC Report
Tue 4 Jul 2023
SRR2518207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2518207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135959467
Total Bases10.3 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA62076594.565815928066267No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA39390682.8972370125575733No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA29081122.1389551343269093No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC18619391.3694809497892486No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT15862201.1666859506002623No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCTGTAGGCACCATCAA14840961.09157238752635No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT11955240.8793238355369546No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT9152960.673212406753551No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATC8605610.632954084764101No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCA8352140.6143110284479124No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCCTGTAGGCACCATCAATAGAT8012730.5893469705938168No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7868150.5787129189025138Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA7707200.5668748318938319No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA7567100.5565702901733206No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6744890.49609564885981794No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA6668360.49046676536323874No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA5868410.43162937671710644No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA5854320.430593038438434No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG5687160.41829819765327564No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCA5370960.395041266232678No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCAT5248860.3860606485019539No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC5025450.36962854524871No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA4955950.3645167276214756No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT4614150.33937688208206934No Hit
CGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATCA4355480.32035135883549765No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCCTGTAGGCACCATCAATAGA3759220.2764956411604644No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA3306210.24317615190415537No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGG3299180.24265908603481068No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACCTGTA3182000.23404034086129508No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3123420.22973170378786495No Hit
TGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGCTGTAGGCACCA2811480.20678810104485038No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA2718210.199927968237769No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATCA2631400.19354297704035572No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCTGTAGGCACCATCA2499070.18380992917543582No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA2161530.15898341231361254No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG2148340.15801327023442951No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA2094160.15402825902516962No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1989440.14632596345791793No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCCTGTAGGCACCATCAAT1934910.14231520928218996No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACCTGTAGGCACC1901850.13988360222094723No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTGTAGGCACCA1816550.13360967353601055No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1806330.1328579789151424No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGAACCATCAA1779400.13087724152375502No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA1757250.12924807950298892No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA1649790.12134425328395854No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC1649370.12131336172419682No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTGTAGGCACCATCAATA1603200.11791749669039228No Hit
TCTCCGGGGACAGTGCCAGGTGGGGAGTTTCTGTAGGCACCATCAATAGA1588910.11686644814516668No Hit
TCGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATC1587370.11675317909270709No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCTGTAGGCACCATC1575120.11585217526632403No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1561240.11483128276753249No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA1497970.11017768994342998No Hit
TGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCAT1495780.11001661252467251No Hit
ACTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1449990.10664869699731908No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGAACCATCA1424590.10478049314506359No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTCTGTAGGCACCATCA1391500.10234667954383787No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATAGATCGGAA1382370.10167515587568464No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGCTGTAGGCACCATCAATAGAT1374400.1010889517535399No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)