FastQCFastQC Report
Sun 2 Jul 2023
SRR2518208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2518208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138062582
Total Bases10.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA33102922.397674990606796No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA16205631.1737887098185662No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA11615010.8412858742566469No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT9225500.6682114636969487No Hit
GTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAA8944440.6478540289794088No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT8625710.6247681214595856No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT7889450.5714401314035978No Hit
GGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGA7827800.5669747651105062No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7397090.5357780430326878No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6924100.5015189416057713No Hit
GTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGATCGGA6265860.4538420120232142No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC5660830.41001913175866866No Hit
GGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGATCGG4636540.33582886346425134No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4285890.3104309609391486No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG4034600.2922297947462695No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA4010900.29051318191340214No Hit
CTGTGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA3963970.2871139987806399No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA3944780.2857240494024659No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA3586560.25977784480374266No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3331490.24130288972865943Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA3230660.23399967994224533No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCA3169280.22955386999788255No Hit
GTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAGATCGGAAG3111840.22539343788311883No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA2684590.19444732679271493No Hit
GTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGA2652930.19215416382695205No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG2583140.18709920983514564No Hit
CTGGGTCGGGGTTTCGTACGTATCAGAGCAGCTCCTGTAGGCACCATCAA2518570.18242234525209733No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACCTGTA2386990.17289188463822877No Hit
TTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAA2317280.1678427251201198No Hit
GGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAGATCGGAA2296870.1663644100180598No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA2115080.15319719284983385No Hit
TCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGAT2055590.14888827734657317No Hit
CTGGGTCGTGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1969700.14266718552315644No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1964340.1422789557854278No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCTGTAGGCACCATCAA1869000.13537339175650068No Hit
CTGTGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA1753340.1269960314084232No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG1639840.11877512185017661No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1638000.11864184895513544No Hit
CTGGGTCGGTGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1628940.11798562480890007No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT1606770.11637983128549631No Hit
CTTGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1586250.11489354878210231No Hit
GCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAA1511230.1094597810723256No Hit
TGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGA1511150.10945398659862815No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATC1440580.10434253648827166No Hit
GGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAA1433840.10385435207926214No Hit
GTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAG1421430.10295548434694637No Hit
TCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGA1395470.10107517763212627No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTGTAGGCACCA1387280.10048196838735059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)