FastQCFastQC Report
Tue 4 Jul 2023
SRR2518209.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2518209.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66623108
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT30421274.566173946733317No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA25641543.848745693461194No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA19916582.989440240464315No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA10983531.6486066666238388No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT10182291.5283420881535579No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC10089801.5144595175595832No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT8724111.3094720828695052No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA8392121.259641024252426No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA7236381.0861666795851073No Hit
AGAGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA6633840.9957265878379615No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA5967880.8957672764230693No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA5607650.8416974482787564No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG5493430.8245532466002637No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA4511690.6771959663004614No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT4285320.6432182659506067No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG3142980.471755235435729No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC3085380.46310958654165457No Hit
CGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATCA2982500.44766749698918284No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA2881720.43254061338597416No Hit
TAGAGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA2806210.4212067080388984No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCTGTAGGCACCATCAA2645950.3971519911679894No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA2645240.3970454215375242No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA2579580.38718998219056366No Hit
ACGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT2499190.3751235982566289No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA2156460.3236804863561754No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2069310.3105994394617555No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT1919150.288060713108731No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCCTGTAGGCACCATC1851380.2778885668317966No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1756910.2637088020570881No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGAACCATCAAT1576160.2365785757097973No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATAG1493130.22411593286821743No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC1455620.21848575422209363No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1428640.2144361082644178No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1388610.20842768247917826No Hit
AGCGGGCCCCCGGTGGGGAGGGGGGCCCGGACACCTGTAGGCACCATCAA1343740.2016927820299227No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1334990.20037942390799302No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1330060.19963944041758003No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATCG1253000.18807288306033398No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACAATCAAT1220620.18321270751883867No Hit
AGAGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA1054000.1582033669158755No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA1023180.15357734436526138No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGAACCATCA1020200.15313005211344988No Hit
CAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA1017840.15277582066570655No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT1010010.15160055276916834No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAATA1006640.15109472226963655No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAATA1005750.1509611349863774No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCCTGTAGGCACCAT946790.14211135271563732No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA930440.13965724925351725No Hit
TCGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATC922590.13847897939555748No Hit
TAGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT883140.13255761049154297No Hit
CGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAATA792060.11888667817778781No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA771900.11586070106486175No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA754390.11323248384029158No Hit
ACTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC690250.10360519356136912No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG686730.1030768483511757No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGAACCATCAA684410.10272862082627549No Hit
CCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG668490.1003390595347188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)