FastQCFastQC Report
Tue 8 Aug 2023
SRR2518210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2518210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59972999
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA32505735.420060784353972No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT25876384.314671674164568No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA24422174.072194221936442No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC11993391.999798275887454No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA10773671.7964200856455421No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA10045111.6749387503533049No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT9370641.5624764737878125No Hit
AGAGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA8740131.4573441624955257No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT7422651.2376653033475948No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA4958760.826832088220234No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA4388970.7318243331469884No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA4373710.7292798547559711No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG4079680.6802527917605055No Hit
CGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATCA3851360.6421823260831094No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA3732190.6223117173113187No Hit
TAGAGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA3648410.6083420974162056No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC3475670.5795391356033405No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCCTGTAGGCACCATC3471910.5789121867992628No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT3377660.5631967812715185No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG2559920.4268454208868228No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCTGTAGGCACCATCAA2462470.4105964419087996No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA2392080.39885949341969706No Hit
ACGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT2170430.36190119490272615No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2043320.34070665700743097No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA2028300.33820219662518464No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA1918670.31992230370203767No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT1821700.3037533607415564No Hit
AGCGGGCCCCCGGTGGGGAGGGGGGCCCGGACACCTGTAGGCACCATCAA1734770.289258504481325No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCCTGTAGGCACCAT1718110.28648058770581075No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1585940.2644423367922621No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA1555310.25933503842287425No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1492050.24878695827767425No Hit
TCGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATC1385800.2310706523113843No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGAACCATCAAT1377650.22971170743020536No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1265710.21104664117263836No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1264970.2109232523122614No Hit
CAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA1257990.20975939522384066No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGAACCATCA1224460.20416854591513758No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1087080.18126157072785373No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACAATCAAT1053890.17572741359824276No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC1048860.1748887028310857No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATCG884080.14741300497578919No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGAACCATCAA875530.14598736341332538No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAATA861690.14367965824086937No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATAG827780.13802544708494566No Hit
ACTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC822660.13717172956449952No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG788580.13148917231902976No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCA778780.1298551036275508No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTCTGTAGGCACCATCA771390.12862288244081307No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGGCACCATCAAT766100.12774081883082086No Hit
TAGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT749120.12490954471027871No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCAT733500.12230503930610508No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCTGTAGGCACCATCAA702670.11716439259607478No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGACCGGACACCTGTAGGCACCATCAA666720.11117002836559832No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGAACCATC650930.10853717687187862No Hit
AGAGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA646110.1077334818623961No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA642450.10712320722863967No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA635000.10588098153970922No Hit
AAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA630740.1051706618840255No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAATA612270.10209094262569729No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAAT601420.10028179514584555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)