FastQCFastQC Report
Sun 2 Jul 2023
SRR2518212.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2518212.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147724635
Total Bases11.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA47945573.245604228434885No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA17255421.1680800565186706No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA17000561.1508276869325147No Hit
GTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAA14422300.9762962013749434No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT12087500.8182453793167267No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA11219650.7594975611210683No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT11184920.7571465652969798No Hit
GGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGA10810750.7318176822708007No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT7950470.5381952712220274No Hit
GTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGATCGGA7525220.5094086033788474No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT7432100.5031049831329758No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT6944720.47011251711672875No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6300710.42651721562893014No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG6041050.408939917164121No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC5884330.39833098927609467No Hit
CTGTGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA5671310.3839109164155322No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA5504460.372616253206515No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA5279110.3573615192889121No Hit
GGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGATCGG4760350.32224483072846993No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4677520.31663777676621097Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGA4340840.293846723669346No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCA4195700.28402168670107053No Hit
TTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAA3957280.2678821985243017No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA3752270.25400435072999167No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA3545760.24002496266110254No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATC3527880.23881460258811946No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCG3414590.23114560411674057No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG3400500.2301918024708607No Hit
TCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGAT3294450.22301290505811708No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACCTGTA3235630.2190311724242879No Hit
CTGGGTCGGGGTTTCGTACGTATCAGAGCAGCTCCTGTAGGCACCATCAA3120770.2112558951321829No Hit
GTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAGATCGGAAG3069080.20775681727018652No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2991500.20250515426895452No Hit
CTGGGTCGTGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2837520.19208170661582613No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA2786680.1886401682427579No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA2422020.16395505055741041No Hit
CTTGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2405800.16285706172162823No Hit
TCAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGG2376080.16084521041463395No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTGTGCTGTAGGCACCATCAATAG2320910.157110559115614No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTGCTGTAGGCACCATCAATAGAT2298410.15558745499692722No Hit
CTGGGTCGGTGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2286590.15478731763324377No Hit
TGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGA2224670.15059573509861776No Hit
GGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAATAGATCGGAA2027720.13726349704637958No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1958580.13258316732344608No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1890790.12799422384763381No Hit
GGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAA1839150.12449853066145669No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAATA1795150.12152001594046924No Hit
ATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGA1794740.12149226159875096No Hit
GTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAG1718340.11632047694685452No Hit
CTGTGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA1702420.11524279616598816No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCCTGTAGGCACCA1696160.11481903475341132No Hit
CTGGTTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1669840.11303734140212972No Hit
TTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGATCGGA1656160.11211129409796816No Hit
TCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGA1627860.11019556758424212No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATA1578530.10685624642091686No Hit
TAGTCGGCATTGGCAATTTTTGACAGTCTCTCTGTAGGCACCATCAATAG1532410.1037342214451909No Hit
CCGCCTGGATACCTCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1485740.10057496503545262No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1485370.10054991843438978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)