FastQCFastQC Report
Fri 29 Sept 2023
SRR2535267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2535267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9155023
Total Bases457.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA1937522.1163464034989317RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1522281.6627811858036838No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA1457921.5924809801133213No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1406791.5366318577244427RNA PCR Primer, Index 1 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1316291.4377790203257819No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCC1054381.15169563200442No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC1033111.12846248447437No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC766770.83754022245493No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA653340.7136410252601222No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC624980.6826634952200556No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC601370.6568743737727365No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG547630.5981743573992113No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG512890.5602279753966757No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC475680.5195836209259114RNA PCR Primer, Index 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT347540.3796167415417744No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA345800.3777161455520101No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCG332280.36294829625223224No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT328680.3590160286872026RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA287810.31437386885865826No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAA286450.31288834555631373RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGC268660.29345639000579243No Hit
TTCCCGCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC237870.25982457935933095RNA PCR Primer, Index 1 (100% over 38bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC228060.24910915024462527No Hit
CGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGTGGAATTCTCGGGTGC213390.23308515991712964No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG203740.22254449824975864No Hit
ACCCCCCGTCCTTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC182930.19981380713079586RNA PCR Primer, Index 1 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC174150.190223443458307No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG170760.1865205581679041Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA166750.18214044901907947No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC163650.17875432972697064No Hit
TTCCCGCTCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA160230.17501867554019251RNA PCR Primer, Index 1 (100% over 37bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG158310.17292146617217674No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG154760.16904381343443922No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGA152530.16660799213721256RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT140280.15322735945065347No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA139980.152899670486901No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC137510.1502016980186724No Hit
GCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA134930.14738357293040116RNA PCR Primer, Index 1 (100% over 34bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT133150.1454392850788032No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC122780.13411216989842625No Hit
CGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG122630.13394832541655RNA PCR Primer, Index 1 (100% over 29bp)
AATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAGG119770.13082435729544317Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCTGGAATTCTCGGGTGCCA110540.12074246017732561No Hit
CTTCCAGCTGATGGGGCTCTATGGCCGAGAATGGAATTCTCGGGTGCCAA107720.11766218391805242No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTTGGAATTCTC105630.11537928413724358No Hit
CCCCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA104040.11364253262935549RNA PCR Primer, Index 1 (100% over 37bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC102220.11165455291592385No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA100980.11030010519908033No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA100680.10997241623532787No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG99390.10856335369119226Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC97900.10693583183788835No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG93200.10180203807243302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)