FastQCFastQC Report
Fri 29 Sept 2023
SRR2535268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2535268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8833985
Total Bases450.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA1872932.120141702753627RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1409451.5954860688579389No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA1360241.5397807444771527No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1358601.53792427766178RNA PCR Primer, Index 1 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1253271.4186915644525093No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCC972601.100975380872845No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC959631.0862934451439525No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC717120.8117740747805209No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA599700.6788555787676797No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC580660.6573024518379871No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC564430.6389302223175611No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG521150.5899376102630919No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG480400.5438089378689233No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC477660.5407072798968982RNA PCR Primer, Index 1 (100% over 24bp)
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT331390.37513081582094604RNA PCR Primer, Index 1 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT324380.36719555217718847No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA317060.35890937102564696No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCG298350.3377298014429501No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAA269200.3047322357916614RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA264920.29988731020032294No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGC248450.28124340260935465No Hit
TTCCCGCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC220270.24934386915984125RNA PCR Primer, Index 1 (100% over 38bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC211870.2398351366908592No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA206060.23325826339981331No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA201040.227575663757636No Hit
CGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGTGGAATTCTCGGGTGC196990.22299109631723396No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG188480.2133578447325867No Hit
ACCCCCCGTCCTTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC179550.20324915652449038RNA PCR Primer, Index 1 (100% over 33bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG158630.1795678847088828Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC157890.1787302106580439No Hit
TTCCCGCTCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA155500.1760247498722264RNA PCR Primer, Index 1 (100% over 37bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA152380.17249293495517595No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC149190.16888188060088397No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG144720.16382187653703284No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGA140130.15862603343791054RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT139930.1583996350457919No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG137490.15563757466194475No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA136620.15465274165622875No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC129810.14694387640458978No Hit
GCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA124160.14054812182723878RNA PCR Primer, Index 1 (100% over 34bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT123970.1403330433547261No Hit
CGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG118130.13372221030486242RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA118040.13362033102840903RNA PCR Primer, Index 1 (100% over 37bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC117340.13282793665599388No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAGG108700.12304752611646953Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCTGGAATTCTCGGGTGCCA102260.11575749789024999No Hit
CTTCCAGCTGATGGGGCTCTATGGCCGAGAATGGAATTCTCGGGTGCCAA98680.1117049666713267No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGTGGAATTCT97810.1107201336656107No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT97290.11013149784610228No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC97190.11001829865004298No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC95000.10753923625634412No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTTGGAATTCTC94100.10652044349181032No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG91600.10369046359032758Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)