FastQCFastQC Report
Fri 29 Sept 2023
SRR2535269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2535269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9121584
Total Bases456 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA1936442.1229207558687175RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1520201.6665965034143193No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA1450131.5897787051020962No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1404071.5392830894283274RNA PCR Primer, Index 1 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1339561.4685607236637845No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCC1045971.1466977665282696No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC1031011.130297106292065No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC766850.8406982822281744No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA651010.7137027954793816No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC617140.6765710867761564No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC604270.6624616952494217No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG557410.6110890389213102No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG514960.5645510692002617No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC473030.5185831759045358RNA PCR Primer, Index 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT345520.378793858610522No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA338930.37156923622037574No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT328470.3601019296648477RNA PCR Primer, Index 1 (100% over 25bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCG327970.35955377925588367No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAA288620.31641434207041236RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA288530.3163156749967988No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGC267470.2932275797712327No Hit
TTCCCGCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC234590.2571812088777563RNA PCR Primer, Index 1 (100% over 38bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC226990.24884932266150267No Hit
CGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGTGGAATTCTCGGGTGC211390.23174702990182405No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG194870.21363614038965162No Hit
ACCCCCCGTCCTTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC194590.21332917616063174RNA PCR Primer, Index 1 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC174180.19095367646671896No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG169160.1854502463607198Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA163670.17943155487029444No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC162260.1778857707170158No Hit
TTCCCGCTCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA161380.17692102599723908RNA PCR Primer, Index 1 (100% over 37bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG156390.1714504849157778No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGA151210.16577164667891015RNA PCR Primer, Index 1 (100% over 22bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG150190.16465341984462348No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT143950.15781250274075204No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA141610.15524715882680026No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC137300.15052210230153007No Hit
GCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA133350.14619171407071402RNA PCR Primer, Index 1 (100% over 34bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT132850.14564356366174996No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA124500.13648945183205022No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA124000.13594130142308616No Hit
CGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG123430.13531640995686714RNA PCR Primer, Index 1 (100% over 29bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC122460.13425299816347688No Hit
AATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAGG118670.1300980180635293Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA114340.12535103552190058RNA PCR Primer, Index 1 (100% over 37bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCTGGAATTCTCGGGTGCCA110470.12110835135651878No Hit
CTTCCAGCTGATGGGGCTCTATGGCCGAGAATGGAATTCTCGGGTGCCAA106260.1164929249130414No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC102930.1128422431893408No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTTGGAATTCTC101300.11105527285611798No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC100310.10996993504636915No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG98910.10843511390126977Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGTGGAATTCT91850.10069523012669729No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG91650.10047596996311167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)