FastQCFastQC Report
Fri 29 Sept 2023
SRR2535270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2535270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8711961
Total Bases444.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA1827932.098184323827896RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1370001.5725506576533113No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1324651.5204957873434006RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA1321031.5163405804961707No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1225521.4067096948666322No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCC948091.0882624474558598No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC937181.0757394345544018No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC696920.7999576673954348No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA589390.6765296584775804No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC567370.6512540632355907No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC549950.631258565092291No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG510090.5855053758849472No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG473310.5432875560393349No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC457970.5256795800623992RNA PCR Primer, Index 1 (100% over 24bp)
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT316190.3629378047032121RNA PCR Primer, Index 1 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT315700.36237535957748207No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA307670.35315814659868194No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCG291070.3341038831555835No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAA261260.2998865582616818RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA259560.2979352180295573No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGC241330.27700996365800995No Hit
TTCCCGCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC216550.24856631015680625RNA PCR Primer, Index 1 (100% over 38bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA210440.24155296379311156No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC208570.23940648953777455No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA204570.23481510075630507No Hit
CGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGTGGAATTCTCGGGTGC192930.2214541594022287No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG179730.2063025764233793No Hit
ACCCCCCGTCCTTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC176890.20304269038853595RNA PCR Primer, Index 1 (100% over 33bp)
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC154300.17711282224518682No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC153640.17635524309624434No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG153420.17610271671326352Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCCGCTCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA151800.17424320425676837RNA PCR Primer, Index 1 (100% over 37bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA150240.17245256263199524No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG139410.16002137750616652No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGA137670.1580241233862273RNA PCR Primer, Index 1 (100% over 22bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG137430.1577486400593391No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT134620.15452318944035676No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA132940.15259480615213958No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC127690.14656860837646082No Hit
GCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA120390.13818932385027896RNA PCR Primer, Index 1 (100% over 34bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT118930.13651346694504257No Hit
CCCCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA116530.13375863367616087RNA PCR Primer, Index 1 (100% over 37bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC114680.1316351163647312No Hit
CGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG113600.13039544139373443RNA PCR Primer, Index 1 (100% over 29bp)
AATCAGTTATGGTTCCTTTGGTCGCTCGCTGGAATTCTCGGGTGCCAAGG108930.12503499499136878Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT98720.11331547512666781No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCTGGAATTCTCGGGTGCCA98030.11252346056186432No Hit
CTTCCAGCTGATGGGGCTCTATGGCCGAGAATGGAATTCTCGGGTGCCAA96160.11037698630652733No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC94750.1087585217610593No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC93990.10788615789258009No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGTGGAATTCT93740.10759919609373825No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG92410.10607255932389964Illumina Small RNA Adapter 2 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTTGGAATTCTC92280.10592333918850187No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)