FastQCFastQC Report
Sat 30 Sept 2023
SRR2536136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10311270
Total Bases515.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA5862695.6857108775155725RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA1810801.7561367319447556No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1544331.497710757258805No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1428561.3854355477065385RNA PCR Primer, Index 1 (100% over 23bp)
CCTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGAA1138471.1041025984190114RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG880870.8542788618666759No Hit
CCTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAAC792820.7688868587477585RNA PCR Primer, Index 1 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA719660.6979353658666683No Hit
ACCCCCCGTCCTTTCCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA671250.6509867358724968RNA PCR Primer, Index 1 (100% over 32bp)
CCTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTC589090.5713069292143451RNA PCR Primer, Index 1 (100% over 26bp)
ACCCCCCGTCCTTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC577130.5597079700172723RNA PCR Primer, Index 1 (100% over 33bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCC559490.5426004750142319No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTCGGGTGCCAAGG532090.5160276086262895Illumina Small RNA Adapter 2 (100% over 21bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA455170.4414296202116713No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG436670.4234880863366006No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCG422300.40955187867255927No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG415690.4031414171096286No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAG410290.3979044288433918No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA407930.39561567100851786No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC391080.37927432799257516No Hit
CTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGAAC381820.3702938629286208RNA PCR Primer, Index 1 (100% over 24bp)
TTCCCGCTCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG317830.3082355519737142RNA PCR Primer, Index 12 (100% over 37bp)
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCTGGAATTCTCGGGT317590.30800279693965926No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC311820.302406977995921No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA303970.2947939487570396No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG303630.2944642124587951No Hit
CCTCCCGACCCCTCCACCCGCGCGTTGGAATTCTCGGGTGCCAAGGAACT296750.28779190148255257RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC289540.2807995523344845No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGC287110.27844290761467794No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCA280890.2724106729820866No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC258420.25061898291868995No Hit
CCCCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG255690.24797139440631463RNA PCR Primer, Index 12 (100% over 37bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGA246200.23876787243472436RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGTGC241890.2345879799481538No Hit
CTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAACT232800.2257723830333218RNA PCR Primer, Index 1 (100% over 25bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC230130.22318298327946023RNA PCR Primer, Index 1 (100% over 24bp)
CCCCCGCCGGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT224890.2181011650359267RNA PCR Primer, Index 12 (100% over 36bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC224650.21786841000187174No Hit
CCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT221160.21448376388165571RNA PCR Primer, Index 12 (100% over 38bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG218490.21189436412779417No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC217570.2110021364972501No Hit
CGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGAA214330.20785994353750797RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG209690.20336001287911187Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAA208800.20249687962782473RNA PCR Primer, Index 1 (100% over 23bp)
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT207780.20150767073309106RNA PCR Primer, Index 1 (100% over 25bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTG206510.2002760086778835No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCTGGAATTCTCG205160.1989667616113243No Hit
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC203230.19709502321246558RNA PCR Primer, Index 1 (100% over 26bp)
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC197930.1919550162104183RNA PCR Primer, Index 1 (100% over 27bp)
CTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTCC196840.190897920430752RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA190620.18486568579816065No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC188150.18247024857267824No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGTGGAATTCT179150.17374193479561684No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG178910.17350917976156185No Hit
TGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA174440.16917411725228804RNA PCR Primer, Index 1 (100% over 28bp)
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG170790.16563430110936866No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCA169590.1644705259390938No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA168430.1633455432744948No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC167140.16209448496644932No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGG165610.1606106716243489Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGTGGAATTCTCGGGTGCCAAGG162540.15763334681372906Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACT152470.14786733350983924RNA PCR Primer, Index 1 (100% over 25bp)
AGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCCA148650.14416264921779762RNA PCR Primer, Index 1 (100% over 28bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT148090.14361955413833602No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT143790.13944935977818446RNA PCR Primer, Index 1 (100% over 25bp)
CCCCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA142820.1385086415155456RNA PCR Primer, Index 12 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC136680.13255399189430594No Hit
CGCGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCC134080.1300324790253771No Hit
CGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGTGGAATTCTCGGGTGC132410.12841289191341124No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA132390.12839349566057331No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTTGGAATTCTC131180.12722002236387953No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTGCC130320.1263859834918492No Hit
TTCCCGCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT129150.12525130270083124RNA PCR Primer, Index 12 (100% over 38bp)
CCTCCCGACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG125460.12167269405223605RNA PCR Primer, Index 12 (100% over 37bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA125340.12155631653520856No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT124370.12061559827256972No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGGAATTCTCGGGTGCCAAG120820.11717276339383995No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACATGGAATTCTCGGGTGCCA119910.11629023388971484No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCCA116580.11306075779220212No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGGAATTCTCGGGTGCCA116360.11284739901098507No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAA115720.1122267189201718No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGGAATTCTCGGGTGCCA112680.10927848848880885No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGTG112050.10866750652441454No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTC109910.10659210747075772No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC107580.1043324440151407No Hit
CCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA105300.1021212711916185RNA PCR Primer, Index 12 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)