FastQCFastQC Report
Sat 30 Sept 2023
SRR2536138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10402886
Total Bases520.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA5789875.565638227699505RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA1807531.7375274515168195No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1542031.4823098128730816No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1417911.3629967683967699RNA PCR Primer, Index 1 (100% over 23bp)
CCTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGAA1129521.0857756203422781RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG879060.8450155081964755No Hit
CCTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAAC795940.7651146037743757RNA PCR Primer, Index 1 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA712810.6852040866351895No Hit
ACCCCCCGTCCTTTCCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA661870.6362369057971029RNA PCR Primer, Index 1 (100% over 32bp)
CCTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTC586050.5633532848480701RNA PCR Primer, Index 1 (100% over 26bp)
ACCCCCCGTCCTTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC579480.5570377297223097RNA PCR Primer, Index 1 (100% over 33bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCC550770.5294396189672751No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA536420.5156453699483009No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTCGGGTGCCAAGG524560.5042446874838387Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG431970.41524053998092453No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCG425680.40919414093358325No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAG409920.3940444988054277No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG409110.39326586872142977No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA407830.39203544093437154No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC388420.3733771570696824No Hit
CTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGAAC377130.3625243994791445RNA PCR Primer, Index 1 (100% over 24bp)
TTCCCGCTCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG319930.30753965774497577RNA PCR Primer, Index 12 (100% over 37bp)
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCTGGAATTCTCGGGT317630.30532873281510536No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC307530.29561988855784826No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG302760.29103462250763873No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA301780.2900925762331722No Hit
CCTCCCGACCCCTCCACCCGCGCGTTGGAATTCTCGGGTGCCAAGGAACT295510.28406540262000374RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC287490.2763560035167164No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGC283570.2725878184188503No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCA279990.2691464657019216No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC262290.2521319564590057No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC259730.24967110088488909No Hit
CCCCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG257540.24756591584296897RNA PCR Primer, Index 12 (100% over 37bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGA244090.23463681136177017RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGTGC240050.23075327365886736No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC229800.2209002386453144RNA PCR Primer, Index 1 (100% over 24bp)
CTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAACT229760.2208617877769688RNA PCR Primer, Index 1 (100% over 25bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC226770.21798758536813725No Hit
CCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT225860.21711282811327548RNA PCR Primer, Index 12 (100% over 38bp)
CCCCCGCCGGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT225310.2165841286735239RNA PCR Primer, Index 12 (100% over 36bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTG214610.2062985213910832No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG213830.20554872945834451No Hit
CGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGAA212060.20384727853405296RNA PCR Primer, Index 1 (100% over 23bp)
TCCTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAA208860.20077120906640714RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG208030.1999733535482365Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCTGGAATTCTCG204330.19641664822627106No Hit
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT203700.1958110470498283RNA PCR Primer, Index 1 (100% over 25bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC201230.1934367059294892RNA PCR Primer, Index 1 (100% over 26bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA198860.19115849198001403No Hit
CTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTCC194630.1870923126524697RNA PCR Primer, Index 1 (100% over 27bp)
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC193350.1858618848654114RNA PCR Primer, Index 1 (100% over 27bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC192030.1845930062100075No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA189150.1818245436891263No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG180160.17318271102845884No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGTGGAATTCT178740.171817705202191No Hit
TGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA171280.16464661825574173RNA PCR Primer, Index 1 (100% over 28bp)
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG170440.16383915002048471No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC168370.16184931758360133No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCA166670.1602151556789145No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGG164800.15841757758375896Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGTGGAATTCTCGGGTGCCAAGG161820.15555298789201383Illumina Small RNA Adapter 2 (100% over 21bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA155390.14937201080546303No Hit
TCCTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACT152070.1461805887327805RNA PCR Primer, Index 1 (100% over 25bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT150230.14441184878888416No Hit
AGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCCA146020.14036489489551265RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA143540.13798094105808714RNA PCR Primer, Index 12 (100% over 39bp)
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT142480.1369619930469295RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC135320.130079287613072No Hit
CGCGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCC134180.12898343786522318No Hit
CGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGTGGAATTCTCGGGTGC132890.12774339736107845No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTTGGAATTCTC131520.1264264551202426No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTGCC129680.12465771517634625No Hit
TTCCCGCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT129190.12418669203911299RNA PCR Primer, Index 12 (100% over 38bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT129080.12408095215116267No Hit
CCTCCCGACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG127410.12247562839773501RNA PCR Primer, Index 12 (100% over 37bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA123850.11905350111497905No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGGAATTCTCGGGTGCCAAG122980.11821719472846284No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCCA117970.1134012234681799No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACATGGAATTCTCGGGTGCCA117130.11259375523292287No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGGAATTCTCGGGTGCCA116540.11202660492482568No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAA114890.1104405066055708No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGTG114670.11022902682967016No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGGAATTCTCGGGTGCCA113660.10925814240394444No Hit
CGTCCCCGCTCGGCGGGGGCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA112270.10792197472893579No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTC111330.10701837932281484No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC106270.10215434447709991No Hit
CCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA104940.10087585310460961RNA PCR Primer, Index 12 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)