FastQCFastQC Report
Sat 30 Sept 2023
SRR2536145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10442209
Total Bases522.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA5553985.318778813946359RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA1781901.7064397006418854No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1514021.4499039427385527No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1338761.282065892379668RNA PCR Primer, Index 1 (100% over 23bp)
CCTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGAA1085911.0399236406779446RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG853660.8175090155732374No Hit
CCTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAAC765350.7329387871857382RNA PCR Primer, Index 1 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA697580.6680387262886617No Hit
ACCCCCCGTCCTTTCCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA648480.6210180240598517RNA PCR Primer, Index 1 (100% over 32bp)
CCTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTC573620.5493282120670061RNA PCR Primer, Index 1 (100% over 26bp)
ACCCCCCGTCCTTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC554580.5310945222414146RNA PCR Primer, Index 1 (100% over 33bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCC539350.5165094856844945No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTCGGGTGCCAAGG500830.4796207392516277Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG421810.40394709586831673No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCG417960.40026013652858317No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAG402750.3856942530071942No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG399840.3829074863374215No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA397510.3806761576980503No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC378250.36223178448161686No Hit
CTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGAAC376070.3601441036087288RNA PCR Primer, Index 1 (100% over 24bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA372720.3569359701572723No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCTGGAATTCTCGGGT308110.295062088874107No Hit
TTCCCGCTCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG306190.293223397463123RNA PCR Primer, Index 12 (100% over 37bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC303630.29077180891514426No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA296100.2835606910376914No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG292660.280266368926345No Hit
CCTCCCGACCCCTCCACCCGCGCGTTGGAATTCTCGGGTGCCAAGGAACT288390.2761771958404587RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC277320.26557599067400395No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGC277070.2653365777298654No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCA274240.2626264232022171No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC255980.24513970176233782No Hit
CCCCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG245500.2351035111440501RNA PCR Primer, Index 12 (100% over 37bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGA240590.23040144092116907RNA PCR Primer, Index 1 (100% over 22bp)
CTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAACT225530.21597920516626318RNA PCR Primer, Index 1 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGTGC223310.21385321822231293No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC222420.2130009081411797RNA PCR Primer, Index 1 (100% over 24bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC217800.20857655693349944No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG210890.20195918315751007No Hit
CCCCCGCCGGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT207770.19897130961466103RNA PCR Primer, Index 12 (100% over 36bp)
CCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT205250.1965580271377445RNA PCR Primer, Index 12 (100% over 38bp)
CGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGAA205110.19642395588902692RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCTGGAATTCTCG201940.1933881997573502No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG201580.19304344511779067Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAA201400.19287106779801094RNA PCR Primer, Index 1 (100% over 23bp)
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT198350.1899502298795207RNA PCR Primer, Index 1 (100% over 25bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTG197750.1893756388135882No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC192250.18410855404254023RNA PCR Primer, Index 1 (100% over 27bp)
CTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTCC192210.18407024797147808RNA PCR Primer, Index 1 (100% over 27bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC191900.18377337592074627RNA PCR Primer, Index 1 (100% over 26bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC183050.17529815769824184No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA183050.17529815769824184No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG180490.1728465691502631No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC174330.16694743420668942No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGTGGAATTCT169940.16274334290761658No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG166200.15916172526330397No Hit
TGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA165090.15809873179132883RNA PCR Primer, Index 1 (100% over 28bp)
GCGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCA164990.1580029666136734No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC160490.15369353361917962No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGG158470.15175907703054017Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGTGGAATTCTCGGGTGCCAAGG155830.14923087634043716Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACT145010.138869084118121RNA PCR Primer, Index 1 (100% over 25bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT144580.1384572938542027No Hit
AGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCCA142180.13615892959047265RNA PCR Primer, Index 1 (100% over 28bp)
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT139010.13312317345879593RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC134440.12874670483994335No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA132060.12646749361174442No Hit
CGCGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCC131260.12570137219050107No Hit
CCCCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA130600.1250693220179753RNA PCR Primer, Index 12 (100% over 39bp)
CGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGTGGAATTCTCGGGTGC129780.12428404756120089No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTTGGAATTCTC128530.12308698284050816No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT122740.1175421790542595No Hit
TTCCCGCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT122470.11728361307458987RNA PCR Primer, Index 12 (100% over 38bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTGCC122160.11698674102385807No Hit
CCTCCCGACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG121870.11670902200865738RNA PCR Primer, Index 12 (100% over 37bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGGAATTCTCGGGTGCCAAG119920.11484160104437671No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA119130.11408505614089892No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACATGGAATTCTCGGGTGCCA116440.11150897286196819No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAA113900.1090765373495206No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCCA112600.10783159004000016No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGTG111650.10692182085227368No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGGAATTCTCGGGTGCCA110170.10550449622297352No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGGAATTCTCGGGTGCCA109810.10515974158341401No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA107590.10303375463946374No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTC106650.10213356196950281No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC104600.10017037582756676No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)