FastQCFastQC Report
Sat 30 Sept 2023
SRR2536146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10613787
Total Bases530.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA6038195.6890061954324125RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA1826791.7211481632333492No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA1677871.5808400903466406No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1584271.4926529051317876No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1458631.374278568054927RNA PCR Primer, Index 1 (100% over 23bp)
CCTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGAA1156771.0898748957370257RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG905010.852673979607844No Hit
CCTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAAC796010.7499773643469574RNA PCR Primer, Index 1 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA721990.6802378830477754No Hit
ACCCCCCGTCCTTTCCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA671850.6329974400277677RNA PCR Primer, Index 1 (100% over 32bp)
ACCCCCCGTCCTTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC588290.5542696494663026RNA PCR Primer, Index 1 (100% over 33bp)
CCTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTC581680.5480419005958947RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCC560160.5277663853627362No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTCGGGTGCCAAGG544220.5127481830943094Illumina Small RNA Adapter 2 (100% over 21bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA494780.46616725962184846No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG437160.41187937915091005No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCG420390.39607917513324886No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAG414400.39043557214781116No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA412900.3890223159744962No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG406600.3830866400465734No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC397870.37486148911788036No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC392300.3696135978609708No Hit
CTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGAAC388020.36558110691311213RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCTGGAATTCTCGGGT317200.2988565721170022No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA311740.29371231964613576No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC310210.2922707983493545No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA308610.29076332509781855No Hit
TTCCCGCTCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG306650.288916670364687RNA PCR Primer, Index 12 (100% over 37bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG304580.28696637684551235No Hit
CCTCCCGACCCCTCCACCCGCGCGTTGGAATTCTCGGGTGCCAAGGAACT300760.2833672844574703RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC297070.2798906742711155No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGC286580.2700073027657329No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCA277990.2619140557465493No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGGAATTCTCGGGTGCC274630.2587483619183238No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC263920.248657712840855No Hit
CCCCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG262380.24720676983625167RNA PCR Primer, Index 12 (100% over 37bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGA248740.23435556036690766RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGTGC246330.23208492878178166No Hit
CTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAACT233050.2195729007940333RNA PCR Primer, Index 1 (100% over 25bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC232570.21912065881857248RNA PCR Primer, Index 1 (100% over 24bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC228610.215389662521021No Hit
CCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT228210.21501279420813704RNA PCR Primer, Index 12 (100% over 38bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTG226920.21379739389908617No Hit
CCCCCGCCGGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT225850.21278927116212148RNA PCR Primer, Index 12 (100% over 36bp)
CGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGAA217370.20479966292898097RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG214130.20174702959462068No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG210420.1982515759926217Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAA209220.19712097105396972RNA PCR Primer, Index 1 (100% over 23bp)
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT208620.1965556685846437RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGTGGAATTCT207130.19515183411915088No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCTGGAATTCTCG199280.1877557934788026No Hit
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC196020.18468431672879812RNA PCR Primer, Index 1 (100% over 26bp)
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC195320.18402479718125114RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA193070.1819049129212787No Hit
CTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTCC192040.18093447701560245RNA PCR Primer, Index 1 (100% over 27bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC188700.17778762660302114No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG185080.17437696837142105No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGTGGAATTCTCGGGTGCCAAGG178050.16775350777248496Illumina Small RNA Adapter 2 (100% over 21bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGG171620.16169534964287488Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA171600.16167650622723068RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC170780.1609039261858185No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCA169970.16014076785222844No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG167850.15814336579394328No Hit
TCCTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACT151760.1429838379081849RNA PCR Primer, Index 1 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGGAATTCTCGGGTGCCAAG149440.14079800169345777No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT148900.1402892294710644No Hit
CCCCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA146120.13766999469652066RNA PCR Primer, Index 12 (100% over 39bp)
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT143900.13557837556001454RNA PCR Primer, Index 1 (100% over 25bp)
AGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCCA143430.13513555529237584RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC135790.1279373705162917No Hit
CGCGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCC134280.12651469263515464No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTGCC132720.12504490621490708No Hit
CGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGTGGAATTCTCGGGTGC132230.1245832425316242No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTTGGAATTCTC131080.12349974613208273No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT130020.12250104510294016No Hit
TTCCCGCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT129190.12171904335370588RNA PCR Primer, Index 12 (100% over 38bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA126550.11923171248867158No Hit
CCTCCCGACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG124730.11751696166504944RNA PCR Primer, Index 12 (100% over 37bp)
CACAAGAAAGACGTGGTCCTGACAGACAGACATGGAATTCTCGGGTGCCA121050.11404977318651675No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGGAATTCTCGGGTGCCA120110.11316413265123938No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAATTCTCGGGTGCCAAGGA118550.11169434623099182RNA PCR Primer, Index 1 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGTG118350.11150591207454982No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCCA117690.11088407935829125No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAA115790.10909395487209231No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGGAATTCTCGGGTGCCA115160.10850038727930003No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG114510.10788797627086355No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTC112880.10635223789586129No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC109760.1034126650553662No Hit
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCAA106700.10052962246180369No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)