FastQCFastQC Report
Sat 30 Sept 2023
SRR2536148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9845312
Total Bases492.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA5884475.976925870911963RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA1693851.7204635058797528No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA1596771.6218582001261108No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1491101.514527929637984No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1425651.4480495894899015RNA PCR Primer, Index 1 (100% over 23bp)
CCTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGAA1068581.0853693615804152RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG831920.8449909967302204No Hit
CCTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAAC756990.7688837083070603RNA PCR Primer, Index 1 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA691600.7024663108695793No Hit
ACCCCCCGTCCTTTCCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA638960.6489992394349717RNA PCR Primer, Index 1 (100% over 32bp)
ACCCCCCGTCCTTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC566420.5753195023174481RNA PCR Primer, Index 1 (100% over 33bp)
CCTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTC540490.5489820942190557RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCC530040.5383679054559165No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGTGGAATTCTCGGGTGCCAAGG527790.5360825538083506Illumina Small RNA Adapter 2 (100% over 21bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA474480.48193495543868997No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG410260.4167059408579433No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC407630.4140346187098997No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCG396500.4027297458932739No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA391290.3974378871893547No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAG383690.38971847717979885No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTTGGAATTCTCGG375310.38120681193242023No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC369930.3757422822151294No Hit
CTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGAAC360390.36605239122945016RNA PCR Primer, Index 1 (100% over 24bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA317370.32235646772799076No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCTGGAATTCTCGGGT300950.30567847925997677No Hit
TTCCCGCTCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG300610.30533313723323346RNA PCR Primer, Index 12 (100% over 37bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA292110.2966995865646513No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC291520.29610031657706737No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG285920.29041233025423674No Hit
CCTCCCGACCCCTCCACCCGCGCGTTGGAATTCTCGGGTGCCAAGGAACT277500.2818600365331236RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC273280.27757373255413337No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGC272900.2771877620536556No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCA264840.269001124596153No Hit
CCCCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG260590.2646843492618619RNA PCR Primer, Index 12 (100% over 37bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGGAATTCTCGGGTGCC257000.2610379437441901No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC243270.24709222013482154No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGTGGAATTCTCGGGTGCCAAGGA232490.2361428464633726RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGTGC229630.23323791059135557No Hit
CCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT221740.2252239441472246RNA PCR Primer, Index 12 (100% over 38bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC221180.22465514551494153RNA PCR Primer, Index 1 (100% over 24bp)
CTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAACT216160.21955627206126124RNA PCR Primer, Index 1 (100% over 25bp)
CCCCCGCCGGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT215250.2186319742838013RNA PCR Primer, Index 12 (100% over 36bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTG214990.218367889204527No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGC214100.21746390566393428No Hit
CGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGAA210850.2141628421730058RNA PCR Primer, Index 1 (100% over 23bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG204140.20734741570404272No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCTGGAATTCTCGGGTGCCAAGGAA198820.2019438286973536RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG196420.19950612027328338Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT195310.19837868012715087RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGTGGAATTCT191850.19486431714911626No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCTGGAATTCTCG186750.18968418674796694No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC183200.1860784097040297RNA PCR Primer, Index 1 (100% over 27bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC183110.18598699563812704RNA PCR Primer, Index 1 (100% over 26bp)
CTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTCC182390.18525568311090598RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA180090.18291954587117198No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC176890.17966926797241162No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG170540.17321949776705908No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGTGGAATTCTCGGGTGCCAAGG166270.16888240819590075Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA163210.16577432995521119RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC162400.16495160336208745No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCA162340.16489066065148572No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGG156610.15907063178901795Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG155990.15844089044613313No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGGAATTCTCGGGTGCCAAG145130.14741025982721523No Hit
CCCCCGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA142580.14482019462664059RNA PCR Primer, Index 12 (100% over 39bp)
TCCTCCCGACCCCTCCACCCGCGCGTGGAATTCTCGGGTGCCAAGGAACT140080.14228091501823406RNA PCR Primer, Index 1 (100% over 25bp)
AGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTCCA140040.14224028654449955RNA PCR Primer, Index 1 (100% over 28bp)
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT135030.1371515702092529RNA PCR Primer, Index 1 (100% over 25bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT133840.13594287311565137No Hit
CGCGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCC130190.13223552488737786No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC129480.1315143694785904No Hit
TTCCCGCTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT125840.12781717836875053RNA PCR Primer, Index 12 (100% over 38bp)
CGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGTGGAATTCTCGGGTGC125430.12740073651297185No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTTGGAATTCTC125390.12736010803923736No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTGCC125070.12703508024936133No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT121870.12378480235060098No Hit
CCTCCCGACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG120410.12230186305929158RNA PCR Primer, Index 12 (100% over 37bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA119060.12093065207075206No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGGAATTCTCGGGTGCCA114950.11675607639453174No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCTGGAATTCTCGGGTGCCAAGGA112500.11426758237829335RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAA111030.1127744859685503No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACATGGAATTCTCGGGTGCCA110650.11238851546807253No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCCA110220.1119517593754266No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGTG109800.11152516040121431No Hit
GTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCAA109590.11131186091410815No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGGAATTCTCGGGTGCCA109260.1109766760057985No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGTGGAATTCTCGGGTG104540.106182516105127No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTC104030.10566450306501206No Hit
CCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA102450.10405967835249914RNA PCR Primer, Index 12 (100% over 39bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC99980.10155087009939351No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA98900.10045390130856188RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)