FastQCFastQC Report
Sat 1 Jul 2023
SRR2536838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43426912
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG24159715.563303695183301No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT13640153.14094403028242No Hit
ACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGTCT7928391.8256858788393706No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA7426461.710105475609226No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT5965081.373590643516168No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA4398941.0129525212384431No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC4280440.9856652943686164No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT3812920.8780085491687736No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC3627680.8353529719082949No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT3576230.8235054797356993No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT3452990.7951267637910797No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTAT3047080.7016570738439796No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC3034660.6987970961416736No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG3025820.6967614920443802No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATG2933110.6754129789380373No Hit
TTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCC2497050.5750005894962091No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT2475960.5701441539292501Illumina Single End Adapter 1 (95% over 21bp)
GCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCG2466040.5678598561187127No Hit
ATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT2368620.545426762096278Illumina Single End Adapter 1 (95% over 21bp)
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT2121630.48855189150911765No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT2098310.4831819494787011No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT2085710.48028052282418787No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT1967970.45316830264145885No Hit
CCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGT1877230.43227342528982954No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1783930.41078905172902924No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT1772030.40804881544421123No Hit
TATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGT1697740.39094191178041854No Hit
GGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGC1670740.3847245689493188No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1625020.3741965350886565No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1602430.3689946915866364No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA1593550.3669498766110747No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1444420.3326094197073004No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1387430.31948622089454576No Hit
GTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCC1330370.3063469030448216No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATTCG1327620.3057136551638763No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA1313910.30255662663741784No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGTA1293580.29787519775755644No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1192640.27463154644751164No Hit
ACCCCTCCCACGGCGCGACCTGTAGGCACCATCAATTCGTATGCCGTCTT1155140.26599634807098416No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCG1125920.259267801495994No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGT1113710.2564561809045967No Hit
GTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGT1109600.25550976316252927No Hit
GCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCT1101060.2535432406522481No Hit
ACCCCTCCCACGGCGCGACCGCTGTAGGCACCATCAATTCGTATGCCGTC1098510.2529560471626442No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATTC1037210.2388403762164807No Hit
TCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT1017550.23431322954761322No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT1010730.2327427748028688No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA963120.22177952694402955No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT958460.22070645962577307No Hit
TCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGTC957420.22046697679079735No Hit
TATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT885580.20392423942093788No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATTCGT884670.20371469194033412No Hit
TTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCG881710.20303308694848027No Hit
TGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGT874540.2013820370188882No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCG873790.20120933305135766No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCT862060.19850824299917988No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATTCGTA853880.19662461839331336No Hit
ATCCGAGTCACGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG850630.1958762345340143Illumina Single End Adapter 1 (95% over 22bp)
TCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGTATGCCGTCT844130.1943794668154162No Hit
ACCCCTCCCACGGCGCGACTGTAGGCACCATCAATTCGTATGCCGTCTTC841020.1936633210300562No Hit
TGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG817820.18832101163444456Illumina Single End Adapter 1 (95% over 22bp)
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC773020.1780048279739531No Hit
TCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCG771780.17771929074763593No Hit
TCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCT768240.17690412802089175No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGC759210.17482477225182394No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGTA734030.16902652438193166No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT728950.1678567428418581No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATG717470.1652132207788571No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA712930.16416778609540555No Hit
TTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGT708270.16309471877714907No Hit
ATCCGAGTCACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA688600.15856526938871454Illumina Single End Adapter 1 (95% over 22bp)
CGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTG683690.15743463408128122No Hit
CCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA673060.15498684318148157Illumina Single End Adapter 1 (95% over 22bp)
ATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGTC671030.15451939110936555No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT664610.15304104514730404No Hit
TTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT660080.15199791318341954No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT646210.14880404114388793No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT639920.14735563053619838No Hit
CCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG631340.1453798971476489No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG630390.14516113878877687No Hit
GGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCC626800.14433446246419732No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC622600.14336732024602625No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATTCGTA620700.1429298035282822No Hit
ATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA620630.14291368449131267Illumina Single End Adapter 1 (95% over 22bp)
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATTCGTATGCCGT603230.13890695244460394No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATG602130.13865365329222581No Hit
ACGAGGCGCCGCCGCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTG596780.1374216983238412No Hit
ATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTG581950.1340067652058705No Hit
GGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTC572330.13179154898234532No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCACCTGTAGGCACCATCAATTCG571430.13158430422130868No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGTAT568370.13087967203378403No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG557550.12838812946220995No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC549470.1265275320520142No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATTCGT548540.12631337913227633No Hit
TCGACTGCATAATTTGTGGTAGTGGGCCTGTAGGCACCATCAATTCGTAT531620.12241717762478714No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATG522580.12033551913615224No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATT522400.12029407018394492No Hit
AGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGTATGC505120.11631497077204107No Hit
ATAGCCGGTCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG504840.11625049462416301Illumina Single End Adapter 1 (95% over 22bp)
TCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA504510.11617450487844956Illumina Single End Adapter 1 (95% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT498860.11487346832305277No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATGC496020.11421949596600374No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGTATGC479730.11046836579124023No Hit
ATGCGTGCATTTATCAGATCAAAACCACTGTAGGCACCATCAATTCGTAT473260.10897850623134338No Hit
GCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCAATTCGTATGCCGT449840.10358553700525609No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATTCGTATGCCGTC443560.10213942911713363No Hit
GCCACCCCTCCCACGGCGCGACTGTAGGCACCATCAATTCGTATGCCGTC443060.10202429313877993No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGTATG436180.10044002207663302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)