FastQCFastQC Report
Sat 1 Jul 2023
SRR2536839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50627792
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG32030166.3265962694956155No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT15443183.050336463419143No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA8220071.623627986778487No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT7489881.4794008792641007No Hit
ACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGTCT5940021.17327257724374No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC5815801.1487366464648507No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA5771861.1400576189457363No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG5115641.010441063675066No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT4644510.9173834798088766No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC4547390.898200340240001No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT4310210.8513525535539848No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT4062790.8024821623664725No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATG3934820.7772055316968989No Hit
TTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCC3328320.6574096693768514No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTAT3248840.641710782093756No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT2989320.5904503992589683No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT2816310.556277469102346No Hit
GCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCG2719260.5371081559314299No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2711150.5355062689678428No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA2674660.528298765231555No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT2611030.5157305694864197No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT2540060.501712577155251No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2334820.4611735783381586No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2229920.4404537333960762No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA2162450.42712706096287983No Hit
GGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGC2160500.42674189701972387No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT2077630.41037341703544966No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC2052220.4053544345761711No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1961080.3873524644329739No Hit
CCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGT1829150.3612936546788373No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT1769580.34952739001534966No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATTCG1728730.34145869920615934No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGTA1626050.3211773485993622No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT1622220.3204208471110097Illumina Single End Adapter 1 (95% over 21bp)
TATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGT1610330.3180723346576126No Hit
GTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCC1564080.3089370360058365No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT1559600.30805214653643204No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1547840.3057293116792452No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGT1518560.2999459269327803No Hit
ATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT1471400.2906308851075315Illumina Single End Adapter 1 (95% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1457100.2878063495243877No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATTC1370090.2706201368607977No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCG1274560.25175105404557246No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA1199430.2369113786356711No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATTCGTA1189180.23488679893446668No Hit
GTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGT1143740.22591149145907846No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATTCGT1143030.22577125227977551No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT1129810.22316003826514894No Hit
CCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG1098710.21701716717173838No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA995690.1966686597748525No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG973300.19224618762753864No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGTA972660.19211977484619513No Hit
GCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCT941750.186014432547246No Hit
TTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGT937270.18512954307784152No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGC931640.18401750564196045No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT924660.18263881624543293No Hit
ACCCCTCCCACGGCGCGACCGCTGTAGGCACCATCAATTCGTATGCCGTC899940.1777561225660404No Hit
TCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCG888410.17547871730214898No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATG881980.17420866388958856No Hit
TGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGT877310.17328624562572273No Hit
TTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCG855260.16893093026849756No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC850460.16798283440842135No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCT810250.16004055638057452No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATG808210.15963761564004214No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT797920.15760513514000374No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCACCTGTAGGCACCATCAATTCG789020.15584720739944574No Hit
TCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGTC779700.15400632127113106No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG774780.15303452301455295No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGTAT756010.14932707316171323No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATTCGTA752870.14870686045324671No Hit
ACCCCTCCCACGGCGCGACCTGTAGGCACCATCAATTCGTATGCCGTCTT750870.1483118205115483No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT738230.14581516808001424No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC735960.14536679774618652No Hit
TCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT727340.1436641755974663No Hit
TGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGC678590.13403507701856718No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT668260.13199469571969483No Hit
TCGACTGCATAATTTGTGGTAGTGGGCCTGTAGGCACCATCAATTCGTAT654450.1292669449222672No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATTC653840.12914645774004918No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATT652340.12885017778377536No Hit
TCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGTATGCCGTCT649520.1282931714659806No Hit
AGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGTATGC644920.1273845796000742No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATG643500.12710410124146834No Hit
GGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCC637270.12587355182307772No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGTATGC637150.12584984942657582No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCG628480.12413735127931316No Hit
TATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT604150.1193316903885518No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATTCGT589710.11647950200948919No Hit
TTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT573930.11336263686948861No Hit
ATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGTC561150.11083833164203566No Hit
CCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGTA559140.11044131650062876No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTCG555320.10968679021198477No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATGC554700.10956432783005823No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT551910.10901324711138893No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATTCGTA535380.10574824199325146No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGTATG526530.10400019025123591No Hit
TGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG522500.1032041847687136Illumina Single End Adapter 1 (95% over 22bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATTCGTAT513970.10151933941736982No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTCGTATG511740.10107886988237606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)