FastQCFastQC Report
Sat 1 Jul 2023
SRR2536840.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536840.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Total Bases816 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT10165326.353325No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG10004866.2530375No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC3328482.0803No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA2904071.81504375No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT2432391.5202437500000001No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC2126671.32916875No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC2059191.28699375No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2001201.25075No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT1991451.24465625No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT1881451.1759062500000002No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT1691351.05709375No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG1587470.99216875No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1587170.99198125No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT1546820.9667625No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1482670.92666875No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT1289150.80571875No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1240020.7750125No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA1142390.71399375No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT1079720.674825No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT1004100.6275625No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC963840.6023999999999999No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT921150.5757187500000001No Hit
ACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGTCT852950.5330937499999999No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT644720.40295000000000003No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATTC625280.3908No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATTCG618020.3862625No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTAT617300.3858125No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC582030.36376875No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCA569150.35571875000000003No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCG565880.353675No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT517920.3237No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATG504190.31511875No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC501000.313125No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA500630.31289375No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA490940.3068375No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG479360.2996No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA474710.29669375No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA465260.2907875No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT457360.28585No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC443250.27703125No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT435200.272No Hit
TCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT430200.26887500000000003No Hit
TTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCC426380.2664875No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA426090.26630625No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT425590.26599375No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT417020.2606375No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCAA413710.25856875No Hit
TTAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT412950.25809375No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAAT406360.253975No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATTC397880.24867499999999998No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT392800.24550000000000002No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT386260.2414125No Hit
CCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG361300.22581250000000003No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT357540.2234625No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGTA347920.21744999999999998No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATT335950.20996875No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGTA326970.20435625No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATTCGT323410.20213125No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA323180.2019875No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA321210.20075624999999997No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG315790.19736875No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCA310300.19393749999999998No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC299800.18737499999999999No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA280690.17543125No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA280220.1751375No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGT278610.17413125000000002No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT263800.164875No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCACCTGTAGGCACCATCAATTCG239070.14941875000000002No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA236460.14778750000000002No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT230180.1438625No Hit
GGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGC229560.143475No Hit
GCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCG228730.14295625No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCG226080.1413No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCACCTGTAGGCACCATCAATT215510.13469375No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT215190.13449375No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATTCGTA215150.13446875No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATT207860.1299125No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG202390.12649375No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT189270.11829375No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT183810.11488125No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGTCTGTAGGCACCATCAATTC182810.11425624999999999No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT180300.11268750000000001No Hit
ATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT180240.11264999999999999Illumina Single End Adapter 1 (95% over 21bp)
TCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATTCGT176560.11035No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC175760.10985No Hit
GTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATGCC174490.10905625No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT174250.10890625No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATTCG170190.10636875No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATTCGTA169130.10570625No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT168310.10519374999999999Illumina Single End Adapter 1 (95% over 21bp)
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT168060.1050375No Hit
TATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGT167990.10499375000000001No Hit
CCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGT166110.10381875No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATG165350.10334375000000001No Hit
AAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT164950.10309375No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATTCG163660.10228749999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)