FastQCFastQC Report
Sat 1 Jul 2023
SRR2536842.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536842.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Total Bases816 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4267852.66740625Illumina Single End Adapter 1 (95% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2713611.6960062500000002No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2662071.6637937500000002No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG2543801.589875No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC1999741.2498375000000002No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1826691.14168125Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1659981.0374875000000001No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT1322100.8263125No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT1251870.7824187499999999No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1186760.741725No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT1128970.70560625No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC921190.57574375No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATTC921110.57569375No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA852050.53253125No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT731130.45695625No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA689190.43074375No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC666400.41650000000000004No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATTCGTA593420.3708875No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA588270.36766875Illumina Single End Adapter 1 (95% over 22bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATTCG553690.34605625No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC544180.3401125No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTCGTATG517240.323275No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT516970.32310625No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC502020.3137625No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT446240.2789No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCG430780.26923749999999996No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG426930.26683125No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT416610.26038124999999995No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT412930.25808125000000004No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT401790.25111875No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC390150.24384375No Hit
ATGCGTGCATTTATCAGATCAAAACCACTGTAGGCACCATCAATTCGTAT373470.23341874999999998No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATTCGTA357560.223475No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC350920.219325No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT334720.2092No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC326690.20418124999999998No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATT322010.20125625No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATTCGTAT316500.19781249999999997No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA311980.1949875No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT308700.1929375No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT285880.178675Illumina Single End Adapter 1 (95% over 22bp)
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGTAT283660.1772875No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT282040.176275No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA278980.1743625No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC270800.16925No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG266970.16685624999999998No Hit
AAATGGATTTTTGGAGCAGGGAGATGGACTGTAGGCACCATCAATTCGTA253620.1585125No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT243320.152075No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC234650.14665625No Hit
ATGCGTGCATTTATCAGATCAAAACCAACTGTAGGCACCATCAATTCGTA232220.1451375No Hit
ACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCGTA221800.138625No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATT219480.137175No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA212250.13265625No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATTCGTAT211830.13239375No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCCTGTAGGCACCATCAATTCGTA207490.12968125No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGTAGGCACCATCAATTCGTATG204890.12805625No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA201600.126Illumina Single End Adapter 1 (95% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATTCGT198230.12389375No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC197080.12317499999999999Illumina Single End Adapter 1 (95% over 22bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT196610.12288125000000001No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT194910.12181875No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTCTGTAGGCACCATCAA192100.1200625No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG191540.1197125No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATTCG190810.11925625000000001No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG186510.11656875No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAATT182530.11408125No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGAACCATCAAT180070.11254375No Hit
TATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGT179190.11199375No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA178420.1115125Illumina Single End Adapter 1 (95% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCCTGTAGGCACCATCAATTCGTAT177770.11110625No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT177230.11076875No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTAT174400.109No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAAT170560.10660000000000001No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATTC169470.10591875No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA168300.1051875No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG167260.1045375No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATTC165110.10319375000000001No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA163220.10201249999999999No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT162110.10131875No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATTCGTA160690.10043125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)