FastQCFastQC Report
Sat 1 Jul 2023
SRR2536844.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536844.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Total Bases816 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4266122.666325Illumina Single End Adapter 1 (95% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2669671.66854375No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2632821.6455125000000002No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG2567611.60475625No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC1966441.229025No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1844711.15294375Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1604291.00268125No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT1299220.8120125No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT1240790.77549375No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1145440.7159000000000001No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT1137610.7110062500000001No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATTC921900.5761875No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC902260.5639125No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA812570.50785625No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT722490.45155624999999994No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA655500.4096875No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC643780.4023625No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATTCGTA596460.3727875No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA588530.36783125Illumina Single End Adapter 1 (95% over 22bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATTCG552060.3450375No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC535620.33476249999999996No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC529140.3307125No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTCGTATG507490.31718125No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT501990.31374375No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCGT449700.2810625No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG431980.2699875No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCG420530.26283125No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC410670.25666875No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT404110.25256875No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT402200.251375No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT397200.24825No Hit
ATGCGTGCATTTATCAGATCAAAACCACTGTAGGCACCATCAATTCGTAT378500.2365625No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATTCGTA357940.2237125No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC354060.2212875No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATT342100.2138125No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT332580.2078625No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC331570.20723125No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT331110.20694375Illumina Single End Adapter 1 (95% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA314730.19670625No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATTCGTAT312350.19521875No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT311000.194375No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT291960.182475No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGTAT286800.17925No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA271800.169875No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC268760.167975No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG261790.16361875No Hit
AAATGGATTTTTGGAGCAGGGAGATGGACTGTAGGCACCATCAATTCGTA254440.159025No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT248070.15504375No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC235340.14708749999999998No Hit
ATGCGTGCATTTATCAGATCAAAACCAACTGTAGGCACCATCAATTCGTA234040.14627500000000002No Hit
ACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCGTA224500.1403125No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATT220650.13790625No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATTCGTAT213010.13313125No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCCTGTAGGCACCATCAATTCGTA210340.1314625No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA206680.12917499999999998No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGTAGGCACCATCAATTCGTATG205960.128725No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATTCGT200790.12549375No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC199300.12456249999999999Illumina Single End Adapter 1 (95% over 22bp)
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG198860.12428750000000001No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT197100.12318749999999999No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT193230.12076875No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATTCG191270.11954375No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTCTGTAGGCACCATCAA188670.11791874999999999No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG187800.11737500000000001No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA186610.11663125Illumina Single End Adapter 1 (95% over 22bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAATT184300.11518749999999998No Hit
TATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGT182930.11433125No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCTGTAGGCACCATCAATTCGTAT182220.11388749999999999No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATTC181930.11370625000000001No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGAACCATCAAT181170.11323125No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT174070.10879375No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAAT173050.10815625000000001No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTAT172150.10759375No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA167810.10488125000000001No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA163810.10238125No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG162430.10151875No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATT161130.10070625No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATTCGTA160560.10035000000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)