FastQCFastQC Report
Sat 1 Jul 2023
SRR2536856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66513563
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGTCT14403362.1654771373471604No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT11383111.7113968169168745No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT10495771.5779894395373169No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG8638051.2986900130429038No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT8564261.2875960351124176No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA7751671.1654269671284938No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT7153531.0754994436247536No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT6719831.0102946973386464No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT6539560.9831919543988343No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA6046580.9090747401398418No Hit
ACCCCTCCCACGGCGCGACCGCTGTAGGCACCATCAATTCGTATGCCGTC5946430.894017660728835No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCT5118730.7695768756215932No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT5035340.7570395830396276Illumina Single End Adapter 1 (95% over 21bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT4884400.7343464670506374No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG4110090.6179326162394878No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA3846740.5783391877533308No Hit
TCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT3104970.46681757222959175No Hit
ACCCCTCCCACGGCGCGACCTGTAGGCACCATCAATTCGTATGCCGTCTT3102510.46644772285015007No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG2835620.42632207208626005No Hit
ACCCCTCCCACGGCGCGACCGCTCTGTAGGCACCATCAATTCGTATGCCG2826330.4249253644704013No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2780530.4180395508206348No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC2663330.40041908445049024No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2592790.3898137286676403No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA2471290.3715467776098538No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG2447510.3679715669419183No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT2333460.3508246881917903No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG2307400.34690669029412846No Hit
ATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTCGTATGC2258680.33958186843787036No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC2226880.33480088865484475No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC2219240.333652250744709No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATG2208860.3320916667777969No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT1986530.29866540152119053No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG1855100.27890552187077994No Hit
ACCCCTCCCACGGCGCGACCGCCTGTAGGCACCATCAATTCGTATGCCGT1746610.26259456285630045No Hit
TGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG1738150.2613226418196842Illumina Single End Adapter 1 (95% over 22bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1729440.26001313446401897No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC1722030.25889907596740835No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC1689960.25407750295980985No Hit
ACCCCTCCCACGGCGCGACTGTAGGCACCATCAATTCGTATGCCGTCTTC1660580.24966035874517803No Hit
GTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT1588870.23887909898917908No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTC1567040.23559706160982533No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT1529490.22995159648867405No Hit
TGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA1469490.2209308799169276Illumina Single End Adapter 1 (95% over 22bp)
GTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT1461240.21969053138831246Illumina Single End Adapter 1 (95% over 21bp)
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT1420750.21360305115514558No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTCGTATG1419930.21347976802866508No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT1367520.20560017210324455No Hit
GTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGT1282370.19279827183517445No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC1276390.1918992070835237No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1236520.1859049409215982Illumina Single End Adapter 1 (95% over 22bp)
ATCCGAGTCACGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG1196600.17990315749586291Illumina Single End Adapter 1 (95% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1180030.17741193626929894No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT1155050.1736563112699285No Hit
ACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATTCGTATGCC1153620.17344131752496855No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT1152660.1732969860598206No Hit
TTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT1149140.17276777068761148No Hit
TTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC1145430.17220998971292517No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT1135030.17064639884048913No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATTC1113460.16740345123294628No Hit
TTTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTG1100940.1655211283749752No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCGTATGCCGTCT1098800.16519938948391624No Hit
TCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGTC1085430.16318927314117876No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGT1047770.1575272700396459No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGTA1043000.15681012307219205No Hit
GCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCG1036240.15579378900510862No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA1014380.152507241267469No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT1008480.15162020413791394No Hit
ATCCCGGACGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA916680.13781850778314192Illumina Single End Adapter 1 (95% over 22bp)
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG914770.13753134830560798No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATT908330.13656312472690718No Hit
GCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCG887450.13342391535993944No Hit
CTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT886570.13329161151688715Illumina Single End Adapter 1 (95% over 21bp)
GCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGT879720.13226174637494612No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATTC877530.13193249022007736No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT874390.13146040605282264No Hit
TTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG872250.13113866716176367No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCCTGTAGGCACCATCAATTCG871560.1310349289211886No Hit
TTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG832300.12513237337774252Illumina Single End Adapter 1 (95% over 22bp)
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT821830.12355825833597278No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCG813080.12224273716925976No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC790840.11889905822666574No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC788860.11860137457979812No Hit
AAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTC786890.11830519438569244No Hit
TATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATTCGTATGCCGT779620.11721218422774916No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT770640.11586208364751112No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATG767910.11545164104349666No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT764310.11491039804919186No Hit
TGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC754890.11349414554742768No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATG753950.11335282098780364No Hit
TTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC751550.11299199232493379No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTAT736780.11077139259552223No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT728750.10956412002767016No Hit
GTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT715210.10752844498797937No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGT714490.10742019638911843No Hit
ACGCCTGTCTGAGCGTCGCCCTGTAGGCACCATCAATTCGTATGCCGTCT699390.10514998271856223No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGC691350.10394120669794821No Hit
TCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTC689580.10367509555908169No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCA684480.10290833465048325No Hit
TCTGAGCGTCGCTTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT683780.1028030929571462No Hit
ATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA675620.10157627550338869Illumina Single End Adapter 1 (95% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCCTGTAGGCACCATCAATT673790.10130114364795041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)