FastQCFastQC Report
Sat 1 Jul 2023
SRR2536858.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536858.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Total Bases816 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT3235102.0219375No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT2989791.86861875No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2371601.48225No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA2276551.42284375No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2229121.3932No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT2193871.37116875No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2006631.2541437500000001No Hit
ACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGTCT1903351.18959375No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT1823061.1394125No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA1703771.06485625No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT1456640.9103999999999999No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG1122700.7016875No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA945540.5909625No Hit
ACCCCTCCCACGGCGCGACCGCTGTAGGCACCATCAATTCGTATGCCGTC920900.5755625No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT914320.57145Illumina Single End Adapter 1 (95% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT907200.567No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG883210.55200625No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT841520.52595No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCT783180.48948749999999996No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA747020.4668875No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG713930.44620624999999997No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC672010.42000624999999997No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC661560.41347500000000004No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT646820.4042625No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC558900.3493125No Hit
TCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT544070.34004375No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC543670.33979375No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG520280.325175No Hit
ACCCCTCCCACGGCGCGACCGCTCTGTAGGCACCATCAATTCGTATGCCG506360.316475No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG495950.30996875No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATG494140.3088375No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT467370.29210625No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC462390.28899375No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT446060.2787875No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTC434470.27154375No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC425350.26584375000000005No Hit
ATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTCGTATGC419360.2621No Hit
TGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG413560.25847499999999995Illumina Single End Adapter 1 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT407700.2548125No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC399610.24975625No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT382400.23900000000000002No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT372000.23249999999999998No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT344690.21543125No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA338210.21138125No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGT336860.21053750000000002No Hit
ACCCCTCCCACGGCGCGACCTGTAGGCACCATCAATTCGTATGCCGTCTT330350.20646875No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTCGTATG323310.20206875No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGTA317680.19854999999999998No Hit
TGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA311590.19474375Illumina Single End Adapter 1 (95% over 22bp)
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT309980.1937375No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATTC305660.1910375No Hit
ACCCCTCCCACGGCGCGACCGCCTGTAGGCACCATCAATTCGTATGCCGT303520.1897No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG276010.17250625No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT264500.1653125No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT263100.16443750000000001No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT262860.1642875No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATTC256110.16006874999999998No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA251860.1574125No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC241880.151175No Hit
GTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT233400.145875No Hit
GTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT233200.14575Illumina Single End Adapter 1 (95% over 21bp)
ATCCGAGTCACGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG230130.14383125Illumina Single End Adapter 1 (95% over 22bp)
GCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCG225830.14114374999999998No Hit
ACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATTCGTATGCC218140.1363375No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATT216070.13504375000000002No Hit
TTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT211380.1321125No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATG211150.13196875No Hit
ATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA211100.1319375Illumina Single End Adapter 1 (95% over 22bp)
GTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGT208660.1304125No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCCTGTAGGCACCATCAATTCG208530.13033125No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGT208040.130025No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT207520.1297No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATG199490.12468125000000001No Hit
GCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCG198160.12385No Hit
ATCCCGGACGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA193710.12106875Illumina Single End Adapter 1 (95% over 22bp)
CTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT193550.12096875Illumina Single End Adapter 1 (95% over 21bp)
TTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC191130.11945625000000001No Hit
GCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGT184210.11513125No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGC182660.1141625No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCG175100.10943750000000001No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG173060.1081625No Hit
TTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG171140.1069625Illumina Single End Adapter 1 (95% over 22bp)
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT170250.10640625No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTAT169640.106025No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGT167000.104375No Hit
ACCCCTCCCACGGCGCGACTGTAGGCACCATCAATTCGTATGCCGTCTTC166740.10421250000000001No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT166270.10391874999999999No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT165760.1036No Hit
TTTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTG164740.1029625No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT163550.10221875000000001No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCA161030.10064374999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)