FastQCFastQC Report
Sat 1 Jul 2023
SRR2536859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Total Bases816 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT3340912.08806875No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT3004771.87798125No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2331141.4569625No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA2306281.441425No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2264231.4151437500000001No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT2221511.38844375No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2047571.27973125No Hit
ACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGTCT1945771.21610625No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT1823151.13946875No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA1728711.08044375No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT1486780.9292375No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG1149320.718325No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA992570.6203562499999999No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT938040.586275No Hit
ACCCCTCCCACGGCGCGACCGCTGTAGGCACCATCAATTCGTATGCCGTC923650.57728125No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT919480.574675Illumina Single End Adapter 1 (95% over 21bp)
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG904100.5650625No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT836040.522525No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCT776260.4851625No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA773520.48345000000000005No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG730930.45683125No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC682170.42635625No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC671160.419475No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT659490.41218124999999994No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC582470.36404375No Hit
TCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT555470.34716874999999997No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC535840.3349No Hit
ACCCCTCCCACGGCGCGACCGCTCTGTAGGCACCATCAATTCGTATGCCG529610.33100625No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG528220.33013750000000003No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATG525240.328275No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT509090.31818125No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG496300.3101875No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC465710.29106875No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC464200.290125No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT455560.284725No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTC449520.28095No Hit
ATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTCGTATGC434900.2718125No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT428250.26765625000000004No Hit
TGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG421400.26337499999999997Illumina Single End Adapter 1 (95% over 22bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC413500.2584375No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT389720.24357499999999999No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT383700.2398125No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT352250.22015625No Hit
ACCCCTCCCACGGCGCGACCTGTAGGCACCATCAATTCGTATGCCGTCTT348940.21808750000000002No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGT346770.21673125No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA340830.21301875No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTCGTATG325850.20365625No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGTA325150.20321875No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATTC317690.19855625No Hit
TGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA315720.19732500000000003Illumina Single End Adapter 1 (95% over 22bp)
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT312100.1950625No Hit
ACCCCTCCCACGGCGCGACCGCCTGTAGGCACCATCAATTCGTATGCCGT309850.19365625No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG286500.17906249999999999No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT284300.1776875No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT272040.17002499999999998No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT269210.16825625No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATTC259580.16223749999999998No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA251500.15718749999999998No Hit
GTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT244850.15303125Illumina Single End Adapter 1 (95% over 21bp)
GTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT244350.15271875No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC243480.152175No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATT242020.15126250000000002No Hit
GCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCG232740.1454625No Hit
ATCCGAGTCACGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG230610.14413125000000002Illumina Single End Adapter 1 (95% over 22bp)
ACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATTCGTATGCC226550.14159375000000002No Hit
ATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA218820.13676249999999998Illumina Single End Adapter 1 (95% over 22bp)
GTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGT214060.1337875No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATG214000.13374999999999998No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGT210200.131375No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCCTGTAGGCACCATCAATTCG209320.130825No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT208480.1303No Hit
TTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT207460.1296625No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATG206120.128825No Hit
GCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCG201660.1260375No Hit
ATCCCGGACGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA194910.12181875Illumina Single End Adapter 1 (95% over 22bp)
CTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT194140.1213375Illumina Single End Adapter 1 (95% over 21bp)
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGC192140.12008749999999999No Hit
TTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC190040.118775No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGT188990.11811875No Hit
GCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGT187990.11749375No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT187010.11688124999999999No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCA178490.11155625No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCG178360.111475No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG177540.11096249999999999No Hit
ACCCCTCCCACGGCGCGACTGTAGGCACCATCAATTCGTATGCCGTCTTC176090.11005625No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTAT173410.10838125No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT172190.10761875000000001No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT170260.1064125No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT169700.10606249999999999No Hit
TTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG168540.1053375Illumina Single End Adapter 1 (95% over 22bp)
TTTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTG163990.10249375000000001No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCCTGTAGGCACCATCAATT160110.10006875000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)