FastQCFastQC Report
Sat 1 Jul 2023
SRR2536860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Total Bases816 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT3343332.08958125No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT3000091.87505625No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2317991.44874375No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA2304421.4402625No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2235201.397No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT2220801.388No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1978911.23681875No Hit
ACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGTCT1886801.17925No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT1814971.13435625No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA1687531.05470625No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT1458270.91141875No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG1147830.7173937499999999No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA946750.59171875No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT933740.5835875No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT926980.5793625Illumina Single End Adapter 1 (95% over 21bp)
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG918480.57405No Hit
ACCCCTCCCACGGCGCGACCGCTGTAGGCACCATCAATTCGTATGCCGTC906980.5668625No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT822970.51435625No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCT785880.49117500000000003No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA772690.48293125000000003No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG717920.4487No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC672340.4202125No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT661180.4132375No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC660050.41253124999999996No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC563690.35230625000000004No Hit
TCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT548950.34309375No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG539710.33731875No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC531550.33221875No Hit
ACCCCTCCCACGGCGCGACCGCTCTGTAGGCACCATCAATTCGTATGCCG506720.31670000000000004No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATG499950.31246874999999996No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG496620.3103875No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT493600.3085No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC462550.28909375No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTC452370.28273125No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT449190.28074375No Hit
ATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTCGTATGC437480.27342500000000003No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC436190.27261874999999997No Hit
TGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG420180.2626125Illumina Single End Adapter 1 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT417350.26084375No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC412930.25808125000000004No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT385720.241075No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT374480.23405No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGT350540.21908750000000002No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT349380.2183625No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA335390.20961875No Hit
ACCCCTCCCACGGCGCGACCTGTAGGCACCATCAATTCGTATGCCGTCTT333530.20845625No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGTA325190.20324375No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTCGTATG323740.2023375No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATTC318180.1988625No Hit
TGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA311560.194725Illumina Single End Adapter 1 (95% over 22bp)
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT311280.19455No Hit
ACCCCTCCCACGGCGCGACCGCCTGTAGGCACCATCAATTCGTATGCCGT305200.19075No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG273490.17093124999999998No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT270080.1688No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT268680.167925No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT265480.165925No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA251860.1574125No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATTC251800.15737500000000001No Hit
GTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT241600.151Illumina Single End Adapter 1 (95% over 21bp)
GTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT238760.149225No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC237990.14874375No Hit
ATCCGAGTCACGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG237130.14820625Illumina Single End Adapter 1 (95% over 22bp)
GCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCG227910.14244374999999998No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATT224910.14056875No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGT218670.13666875No Hit
ATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA218470.13654375Illumina Single End Adapter 1 (95% over 22bp)
ACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATTCGTATGCC215690.13480625000000002No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATG212880.13305No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT209650.13103125000000002No Hit
TTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT208220.1301375No Hit
GTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGT207450.12965625No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCCTGTAGGCACCATCAATTCG204960.1281No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATG203950.12746875No Hit
GCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCG201880.126175No Hit
CTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT196140.1225875Illumina Single End Adapter 1 (95% over 21bp)
ATCCCGGACGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA192530.12033125Illumina Single End Adapter 1 (95% over 22bp)
TTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC191620.1197625No Hit
GCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGT190110.11881875No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGC186550.11659375000000001No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT178620.1116375No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG178190.11136875000000002No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGT177270.11079375No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCG176010.11000624999999999No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTAT173430.10839375000000001No Hit
TTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG172760.107975Illumina Single End Adapter 1 (95% over 22bp)
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT170000.10625000000000001No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT168320.1052No Hit
ACCCCTCCCACGGCGCGACTGTAGGCACCATCAATTCGTATGCCGTCTTC167120.10445No Hit
TTTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTG166060.10378749999999999No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT165300.1033125No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCA164490.10280624999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)