FastQCFastQC Report
Sat 1 Jul 2023
SRR2536861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Total Bases816 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGT3265412.04088125No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT2814021.7587625No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA2223011.38938125No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2192011.37000625No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT2142291.33893125No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2106621.3166375No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1884771.17798125No Hit
ACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCGTCT1822221.1388875No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT1719521.0747No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA1634241.0214No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT1409790.88111875No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG1109690.69355625No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTA941200.5882499999999999No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT884540.5528375No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG882400.5515No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT880140.5500875Illumina Single End Adapter 1 (95% over 21bp)
ACCCCTCCCACGGCGCGACCGCTGTAGGCACCATCAATTCGTATGCCGTC868850.5430312500000001No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT773460.48341249999999997No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTA751310.46956875000000003No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCT737370.46085624999999997No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG705770.44110625000000003No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC638700.3991875No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT636570.39785625No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC636140.39758750000000004No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC551480.344675No Hit
TCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT533530.33345625No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATTCGTATGCCG523930.32745625No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATG511100.31943750000000004No Hit
ACCCCTCCCACGGCGCGACCGCTCTGTAGGCACCATCAATTCGTATGCCG500130.31258125000000003No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC498710.31169375No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG470610.29413125No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT466090.29130625No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC438020.2737625No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC434480.27155No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTC426910.26681875No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT425920.2662No Hit
ATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTCGTATGC424170.26510625000000004No Hit
TGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG409910.25619375Illumina Single End Adapter 1 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT403980.25248750000000003No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC400180.25011249999999996No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT365800.22862500000000002No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT364150.22759375No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGT345920.21619999999999998No Hit
ACCCCTCCCACGGCGCGACCTGTAGGCACCATCAATTCGTATGCCGTCTT333540.20846250000000002No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT333270.20829375000000003No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA326670.20416875No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCCTGTAGGCACCATCAATTCGTA310190.19386875No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTCGTATG306720.1917No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTAT304250.19015625No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATTC303750.18984375No Hit
TGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA299290.18705625Illumina Single End Adapter 1 (95% over 22bp)
ACCCCTCCCACGGCGCGACCGCCTGTAGGCACCATCAATTCGTATGCCGT289440.1809No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG272410.17025625No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT271230.16951875No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT265230.16576875000000002No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT254370.15898125000000002No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATTC243800.15237499999999998No Hit
GTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT237660.1485375Illumina Single End Adapter 1 (95% over 21bp)
GTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT237030.14814375No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA235070.14691875No Hit
ATCCGAGTCACGCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG231540.1447125Illumina Single End Adapter 1 (95% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATT230880.1443No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC228530.14283125No Hit
GCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATTCGTATGCCG222160.13885No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCCTGTAGGCACCATCAATTCG213600.1335No Hit
ATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA213360.13335Illumina Single End Adapter 1 (95% over 22bp)
ACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATTCGTATGCC211600.13225No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGT209280.1308No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTATG205570.12848125No Hit
TTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCT203110.12694375No Hit
GTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATTCGTATGCCGT202230.12639375No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT198760.124225No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATG197100.12318749999999999No Hit
GCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGTATGCCG196090.12255625No Hit
CTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT190170.11885625Illumina Single End Adapter 1 (95% over 21bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTCGT187350.11709375000000001No Hit
GCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGCCGT186070.11629374999999999No Hit
ATCCCGGACGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA185670.11604375Illumina Single End Adapter 1 (95% over 22bp)
TTCTGATCGAGGCCCCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC183980.1149875No Hit
ACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATGC177850.11115625No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCG173870.10866875No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCA172250.10765625000000001No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCG170230.10639375No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT168330.10520625No Hit
ACCCCTCCCACGGCGCGACTGTAGGCACCATCAATTCGTATGCCGTCTTC167910.10494375No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTAT167100.10443749999999999No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT165480.103425No Hit
TTGAGCGTCGCTCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG163770.10235625Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)