FastQCFastQC Report
Sat 1 Jul 2023
SRR2536864.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536864.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Total Bases816 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA3152651.9704062500000001No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2432031.52001875No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2119281.3245500000000001No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA2087591.30474375No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC2046141.2788374999999998No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATTC2007321.254575No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1990951.2443437499999999Illumina Single End Adapter 1 (95% over 22bp)
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG1967601.22975No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1934411.20900625No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA1777361.1108500000000001No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1572180.9826125No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT1286330.8039562499999999No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT1096590.6853687500000001No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT1066880.6668000000000001No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG1023630.63976875No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATTCG772360.482725No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATT759460.4746625No Hit
ACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCGTA758150.47384374999999995No Hit
ATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGCTGTAGGCACCATCAA704030.44001874999999996No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC664600.41537500000000005No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC614790.38424375No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAAT583160.364475No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA573090.35818125Illumina Single End Adapter 1 (95% over 22bp)
AAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATT564160.3526No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGTAT544310.34019375No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC532360.332725No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAATT524370.32773125No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT503680.3148No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC469670.29354375No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTC469580.2934875No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT441260.27578749999999996No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATT414690.25918125No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTAGGCACCATCAATTC405660.25353749999999997No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT398740.2492125No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT378940.23683749999999998No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTACTGTAGGCACCATCAAT359520.22469999999999998No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA321800.201125No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT317990.19874375No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC301690.18855625No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCG290520.18157500000000001No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG272990.17061875No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATTCGTA260090.16255625No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATTC258840.161775No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT251070.15691875No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG247910.15494375No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA234170.14635625Illumina Single End Adapter 1 (95% over 22bp)
ATGCTAAGACTTCACCAGTCAAAGCGAACTGTAGGCACCATCAATTCGTA233790.14611875No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGAACCATCAAT230770.14423125No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCG227450.14215625No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATTC225700.1410625No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT219150.13696875No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT214730.13420625No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT211010.13188125No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT203100.1269375No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC200800.1255No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG198000.12375000000000001No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATTCGTAT197050.12315625No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA193190.12074375No Hit
ACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGTAT189230.11826874999999999No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATT187700.11731249999999999No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATTCG187670.11729374999999999No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATTCGT187400.11712499999999999No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT186890.11680625No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT184810.11550625No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGCCTGTAGGCACCATCAATTCG183830.11489375No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATTCG182520.11407500000000001No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATTC182270.11391875No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACCTGTAGGCACCATCAATTC180740.1129625No Hit
ACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATTCGT180670.11291875No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATT180640.1129No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC180220.1126375No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT179560.11222499999999999No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA166040.103775No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATT165340.1033375No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT161610.10100624999999999Illumina Single End Adapter 1 (95% over 22bp)
CCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG160610.10038124999999999No Hit
TCACCTCTAGCATCACAAGTATTAGAGGCACTGTAGGCACCATCAATTCG160220.1001375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)