FastQCFastQC Report
Sat 1 Jul 2023
SRR2536866.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536866.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Total Bases816 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA3206452.00403125No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2459381.5371124999999999No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2227091.3919312499999998Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2156411.34775625No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA2136171.33510625No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC2083061.3019125No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATTC2018171.26135625No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG1978671.23666875No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1973311.23331875No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA1823411.13963125No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1589310.9933187499999999No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT1286130.80383125No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT1116250.69765625No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT1077630.6735187499999999No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG1022900.6393125000000001No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATTCG782650.48915625No Hit
ACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCGTA761200.47574999999999995No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATT721650.45103125000000005No Hit
ATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGCTGTAGGCACCATCAA714770.44673124999999997No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC668370.41773124999999994No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA607310.37956875Illumina Single End Adapter 1 (95% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC597700.37356249999999996No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAAT586680.366675No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATT557370.34835625000000003No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGTAT542800.33925No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAATT522580.3266125No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC520480.3253No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT515070.32191875No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC472800.29550000000000004No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTC469790.29361875No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT448910.28056875No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTAGGCACCATCAATTC410610.25663125No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATT409340.2558375No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT399530.24970625000000002No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT382350.23896874999999998No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTACTGTAGGCACCATCAAT357940.2237125No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA329460.20591250000000003No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT317910.19869375No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCG300260.1876625No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC293830.18364375No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG275590.17224375No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATTC262200.163875No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATTCGTA260700.16293749999999999No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA252290.15768125Illumina Single End Adapter 1 (95% over 22bp)
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT250300.1564375No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG249470.15591875No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAACTGTAGGCACCATCAATTCGTA240030.15001875No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATTC233400.145875No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGAACCATCAAT229240.14327499999999999No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT225440.1409No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCG222970.13935625000000001No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT217960.13622499999999998No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT213550.13346875No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG204070.12754375No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT204010.12750625No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC201280.1258No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATTCGTAT194300.12143749999999999No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA192880.12054999999999999No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATT191660.11978749999999999No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT191450.11965624999999999No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATTCGT190020.11876250000000001No Hit
ACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGTAT187630.11726874999999999No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT186760.11672500000000001No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATTCG186080.1163No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC184000.11499999999999999No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT183210.11450625No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGCCTGTAGGCACCATCAATTCG182960.11435No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATT182190.11386874999999999No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACCTGTAGGCACCATCAATTC181450.11340625000000001No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATTC181380.1133625No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATTCG180790.11299375No Hit
ACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATTCGT176460.1102875No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA168870.10554374999999999No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATT162930.10183124999999998No Hit
CCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG160730.10045625No Hit
TCACCTCTAGCATCACAAGTATTAGAGGCACTGTAGGCACCATCAATTCG160400.10024999999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)