FastQCFastQC Report
Sat 1 Jul 2023
SRR2536867.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2536867.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6888123
Total Bases351.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1263131.8337796813442502No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT992901.441466710161825No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATTC858291.2460433705960245No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT849201.2328467421386058No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTC835361.2127541857193898No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG823881.1960878166664561No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA808331.173512726180993No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA799151.1601854380358771Illumina Single End Adapter 1 (95% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC754631.0955524458549883No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA687090.9974996091097676No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT633440.9196119174991503No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT536090.7782816886399967No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT440180.6390420147839985No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT439490.6380402905116531No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCG430180.6245242717065302No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATT350020.5081500431975445No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATTCG329380.47818542148565No Hit
ACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCGTA322580.4683133561929716No Hit
ATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGCTGTAGGCACCATCAA285170.4140024793401627No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC274870.39904920397037047No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC265270.38511217061600095No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAAT257020.3731350325770896No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATT248610.36092561064893874No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA241320.35034217594546435Illumina Single End Adapter 1 (95% over 22bp)
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGTAT235160.34139924620974393No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAATT226500.32882688070465643No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC217850.31626903294264636No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATT201560.2926196294694505No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTC200850.29158886971095027No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATTC197250.2863624822030617No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT182800.26538434345612005No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATT179600.2607386656713302No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTAGGCACCATCAATTC168130.24408681436147409No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT163660.23759738320584578No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATT157560.22874155992859013No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTACTGTAGGCACCATCAAT156770.22759465822547015No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC133810.19426192011960297No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT133310.19353603296572955No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA130780.18986304396713008No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTCG120570.175040428285035No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATTCGTA114400.16608298080623704No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATTC112150.16281648861380668No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCG108590.15764817207822798No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG107880.15661741231972776No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT102180.1483422987655708No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGAACCATCAAT98020.14230291764534403No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCG97950.14220129344380175No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA95150.13813632538211063Illumina Single End Adapter 1 (95% over 22bp)
ATGCTAAGACTTCACCAGTCAAAGCGAACTGTAGGCACCATCAATTCGTA94860.13771531083286404No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT91820.1333019169373137No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT87200.12659471963552335No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATTC86070.12495421466776943No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT85760.12450416463236791No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG84650.12289269515076895No Hit
ACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGTAT84620.12284914192153652No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATTCGT83400.12107797726608541No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATTCG83330.12097635306454313No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC83150.12071503368914871No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATTCGTAT82910.12036660785528946No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT81960.11898742226292998No Hit
CGCACCGCACGTTCGTGGGGAACCTGGCGCCTGTAGGCACCATCAATTCG79710.1157209300704996No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT79330.1151692558335558No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT79320.11515473809047835Illumina Single End Adapter 1 (95% over 22bp)
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA77020.11181565718266064No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACCTGTAGGCACCATCAATTC76420.11094459259801254No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATTCG76190.11061068450723079No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATT76020.11036388287491382No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT75610.10976865540873763No Hit
ACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATTCGT75060.10897017953947687No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT72880.10580531154858877No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATT72430.10515201311010272No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATTC71480.10377282751774322No Hit
TCAACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATT71310.10352602588542625No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATTC71080.1031921177946445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)