FastQCFastQC Report
Wed 27 Sept 2023
SRR2724708.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724708.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997801214
Total Bases50.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT139079901.393863808227437No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTAT113569161.1381942455724452No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA95743120.9595410253730159Illumina Single End Adapter 1 (95% over 22bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA87299020.8749139485412573No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC84646280.8483280919319467No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT84336810.8452265723541204No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA84290290.8447603472248331No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT76082790.7625044841847627No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA71703370.718613777914285No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTA61941630.6207812651548849No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA60144980.6027751736128876No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA59397900.5952879107240693No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT57033850.5715953157779983No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT54803970.5492473774440607No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC53276970.5339437279939008No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC50962410.5107471236249669No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG50334800.504457193414479No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT45400640.45500686271965185No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTA44664640.44763064399318314No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA35586110.3566452866632812No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA35165180.35242671091799255No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT32799320.3287159760861947No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT32611120.3268298288520623No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC32490160.3256175633396253No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC29628750.29694040841285246No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT28579850.28642829452400326No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATT28394950.28457522000970426No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT26037710.26095087513092563No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC25930370.2598751097530735No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG25805200.2586206514677582No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATT25301370.25357124891211047No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCGT25233680.25289285727407423No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA25194380.25249899124696795No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCG24806990.2486165545996219No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCA23735450.23787754180864326No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATCA23356820.23408289819940026No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGT22496850.225464247631192No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT22436170.22485611046771087No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT22377750.2242706231062974No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT22146420.22195222544597945No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG22099650.2214834948076141No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATT21339060.21386083420820534No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTA20715890.20761540183894786No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT20100930.20145225038782125No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTATGC19507410.19550397139524828No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT18810030.18851480371119292No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTATG18606110.18647111006621805No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCAA17649650.1768854332141552No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC17578240.17616975960103412No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC17469440.17507936205016483No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA16775600.168125672374658No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATTCG16647130.1668381413695093No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATT16563750.16600250398171995No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA16454230.1649048905647052No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAA16224590.16260343014575646No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGT15771680.15806434967917368No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATTCGT15706860.15741472128535616No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATTC15156110.15189508478589603No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT14339380.1437097870678678No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCTGTAGGCACCAT14147100.14178274992557785No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAAT14106620.14137705789562208No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAA13969330.14000113253019153No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA13846360.1387687227247651No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA13669570.13699692692496565No Hit
AAATAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCA13641530.13671590902674527No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATTCGTA13170440.13199462793998967No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC13155870.13184860687090724No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAA13142230.13171190629559607No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT12777890.12806047758526778No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC12653760.12681644221771812No Hit
TTCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCA12249780.12276773998793711No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT12243770.12270750754969516No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCGT12235380.12262342266502793No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATTCGTA12163740.12190544398355482No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT11993860.12020290045467914No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTAT11729770.11755618088474283No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA11699420.11725201208263915No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG11328390.11353353594937597No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATC11304900.1132981183163804No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA11286410.11311281086495051No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATTCGT11284110.11308976018143028No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCG11268010.11292840539678878No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA11203350.11228038052878135No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA11192720.1121738462827727No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA11055620.11079982510424165No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATTCGT10968100.10992269648611593No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA10958620.1098276875818674No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTC10717380.10740997154168624No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCA10654330.10677808215214299No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAAT10648390.10671855125644295No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATTCGT10600210.10623568954687601No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC10372780.1039563778281793No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT10368010.10390857271496563No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCACCATCAA10350780.1037358930292903No Hit
GACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTAT10321910.10344655684093003No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT10241340.1026390813751806No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCAAT10173650.10196068973714438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)