FastQCFastQC Report
Sat 23 Sept 2023
SRR2724709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369186400
Total Bases18.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA78952602.1385565665474133Illumina PCR Primer Index 6 (96% over 29bp)
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT77600612.101935770115042No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA74798182.0260275026382337No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGCTGTAGGCACCA55095851.4923586025920783No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA34549420.9358259134139285No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA32420540.8781618174450629No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC29668100.8036076085142898No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA26951310.730019036454214No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT26902830.7287058786564186No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA21353130.5783834399100292No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAT20997420.5687484696077646No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC20783510.5629543775176984No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA20366470.5516581867587755No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT18342990.4968490171902324No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCAC18059270.48916401037524676No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT15675890.4246063776997202No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATC14441040.39115850421358966No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA13798650.3737583507951539No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCA13200280.3575505489909704No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA12313350.3335266412847277No Hit
CCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT11246810.3046377114649944No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATC9841930.26658430538069655No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT9400170.2546185341605216No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATATC9131140.24733142932675742No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT8946300.24232474435678023No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATC8766850.2374640560974077No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGCTGTAGGCACCA8623270.23357496375814493No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCAA8378550.22694633388445512No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC8096100.21929572703653222No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATATCT7976700.21606158840087286No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT7820130.21182064128039388No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT7793600.21110203409443035No Hit
CCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGCTGTAGGCACCA7754250.21003617684725115No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCTGTAGGCACCATCAA7591880.20563812751498972No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCA7252060.1964335630998325No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT7104260.19243016535820387No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGC7014450.18999751886851737No Hit
GCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATATCTCGTAT6971440.1888325247083858No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT6959460.18850802738129033No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATA6835110.18513981013385108No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT6700240.18148664197814438No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATC6636900.17977097747912707No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCACC6414420.17374475332785824No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA6344000.17183731578411338No Hit
TGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGCTGTAGGCACCAT5813240.15746083821072498No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT5572000.15092646966410464No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT5162360.13983071965814559No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAT5107850.13835422973327294No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC5077190.13752375493788505No Hit
GCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCAT4982240.1349518833846534No Hit
AAATAGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATAT4890460.13246587631613732No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCCTGTAGGCACCATCA4814670.1304129837935525No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT4787060.1296651230922916No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA4491540.12166049453609341No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCT4448970.12050741847478671No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT4448210.12048683266772557No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA4237200.11477129168355063No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATA4145500.11228745154209364No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT4132530.11193613849264221No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCTGTAGGCACCATCAAT4115920.11148623026200316No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA3967560.10746766403096106No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA3966980.10745195380978281No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3744850.1014352099643974Illumina PCR Primer Index 6 (96% over 29bp)
TGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCATC3695430.10009659077365796No Hit
ACGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCCGTCCTGTAGGCAC3694760.10007844275953827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)