FastQCFastQC Report
Sun 2 Jul 2023
SRR2724710.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724710.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65048484
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA9021041.3868178695755615No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT7103631.0920515841691254No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA7069731.0868400868496797No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA6343810.9752433277307432No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC6246830.9603344483785357No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA5612470.8628133439666328No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG4389220.6747613057361952No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT3718070.5715844200150768No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAT3400480.522760837900542No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTC3210400.4935395573554028No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTC2996980.46073018396554793No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT2976700.45761250946294146No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT2919940.448886710411268No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA2913440.4478874557629967No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT2845610.43745984917957503No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATATC2763790.42488153913010485No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA2756950.4238300157771548No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC2563790.39413524226021934No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT2559400.3934603610439253No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC2553950.39262252445422097No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATATCTCG2412400.3708618328445594No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCA2358130.36251882518891604No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAA2130060.327457285553342No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTCG2079250.3196461888335476No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT1998250.30719393860124394No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC1904640.292803134351294No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT1778890.27347140019435345No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC1733470.2664889161752025No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT1720090.2644319889146071No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAAT1653000.25411814362960405No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT1634720.2513079320956965No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATA1456630.22392989204790692No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCT1444040.22199441265994763No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1394090.2143155250166937No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAT1330280.20450591900035672No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC1274180.19588158272835382No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA1216400.1869989775626439No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC1203100.1849543488207965No Hit
AAACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATATCTC1172060.18018252354659026No Hit
TAACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATATCTC1170030.1798704486333609No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATAT1146570.17626390801052336No Hit
TGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACCTGTAGGCACCATCAA1117660.1718195307979814No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1103630.16966267807255894No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCTGTAGGCACCATCAA1099970.16910002083984002No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1071300.16469253918354193No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAA1055990.1623389101581522No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTC1034840.1590874892641618No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCT1033780.1589245338907514No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATATC1016170.156217322451358No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT1005230.15453550001257524No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT998650.153523946845556No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAT986950.1517252884786677No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC966310.14855227064169552No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA962280.14793273275976732No Hit
TTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATA937530.14412787852211897No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT935720.1438496245354465No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT931090.14313784776290867No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCT928500.14273968321844363No Hit
TGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATA915560.14075039781096205No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT906930.1394236951010265No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA894050.13744363358260586No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCG882320.13564036327118706No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTCG882090.1356050050297867No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAT876610.13476255649555186No Hit
GGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAT849400.13057952280640392No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT848370.130421179377524No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCTGTAGGCACCATCA842260.129481880008149No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCCTGTAGGCACCATCAATATC841880.12942346204409622No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATATCT835660.12846725221144278No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAT805560.123839934532525No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC802630.12338950128338118No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA799310.12287911275534107No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA761610.11708343579536766Illumina PCR Primer Index 6 (96% over 29bp)
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAACTGTAGGCACCATCAATA726700.11171666967672914No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATATC719800.11065592243471808No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT678390.10428990166780829No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA673160.10348588600466077No Hit
TTAACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATATCT668540.10277564654696643No Hit
TACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCT666700.10249278061576347No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCT663960.10207155634864604No Hit
AGTTAAAAAGCTCGTAGTTGGATCTTGGGCTGTAGGCACCATCAATATCT663150.101947033846323No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)