FastQCFastQC Report
Sat 1 Jul 2023
SRR2724711.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724711.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57145331
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC4967530.8692801166905483No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC4584990.8023385147598496No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3534880.6185772202456925No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA2917280.5105018990965333No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2638230.461670263140133No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCA2466880.4316853112636621No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGC2335350.4086685577164651No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCT2269540.3971523062837802No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT2265430.3964330874205628No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC1846240.3230780131451159No Hit
TGGGCCAGCTTTAGCTCAGCGGTTACTTCGACCTGTAGGCACCATCAATA1821020.31866470420829307No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATAT1564910.2738473944616753No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1481440.2592407768186696No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA1469280.25711286894112134No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATATC1393020.24376794667616852No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATC1372760.24022260016308244No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1356670.23740697205866215Illumina PCR Primer Index 6 (96% over 29bp)
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1327300.2322674445616563No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT1278040.2236473177484964No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1244800.21783056957006686No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTCG1121470.19624875390082175No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT995150.17414371088339659No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT976120.1708136050520033No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT906770.15867788043786116No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCAT850520.14883455657995925No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCT837690.14658940377823693No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT813410.14234058772010613No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA756210.13233102105927078No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATC748870.1310465766660797No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGCTGTAGGCACCATCAATA737890.12912515984901723No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATC716970.12546431833599844No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT716840.12544156932086017No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGC703240.12306167235254968No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAT692670.12121200242938483No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAA691490.12100551136889906No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCT664030.11620021940200154No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATC655840.11476703144829103No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCTC651100.11393756735786516No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA643470.11260237516167332No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAAT640210.11203189985897535No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA629710.11019447940550033No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC624730.10932301713328074No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCT616240.1078373314523281No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA611640.1070323663012819No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAT573450.10034940562335705No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)