FastQCFastQC Report
Sat 1 Jul 2023
SRR2724716.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724716.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57170514
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCT7143341.2494797580445054No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTCG6379651.1158986606277495No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA4423820.7737939875789818No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC4230380.7399583638516876No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCT4086940.7148685072168496No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3620180.6332250222553535No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3348740.585746001863828No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT3234720.5658021545861911No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT2758680.48253545525233515No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATATC2495480.43649773727764635No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT2258160.39498682835001275No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCTC2201360.3850516369329826No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCT2149780.3760295036004049No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA2123410.37141698603584355No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC1787140.3125982040322394No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATC1662150.2907355354545177No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATA1575270.275538890554666No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTC1511480.26438104089811054No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATC1491800.2609387069705198No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT1440470.2519603024734044No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATAT1357680.2374790613217156No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAA1343940.23507572452470868No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC1316290.23023931532258046No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1286340.22500060083419926Illumina PCR Primer Index 6 (96% over 29bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT1114330.19491341288273184No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1101000.19258179137588302No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT1100710.1925310659267468No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT1066450.18653846631499588No Hit
TGGGCCAGCTTTAGCTCAGCGGTTACTTCGACCTGTAGGCACCATCAATA956250.16726279564322266No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA933450.1632747258490627No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC911340.1594073476407786No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAAT895730.1566769191545138No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA878220.1536141515187357No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAT860730.15055488218979454No Hit
TTTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATC839840.1469009006985664No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCA820400.14350054645301946No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC816750.14286210545527017No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATATCT813170.14223590853145032No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAT808850.14148027425466211No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATATC798380.1396489106255018No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC794000.13888278142820265No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA766290.1340358773055635No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA754230.13192639828286307No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT746810.13062852644634262No Hit
GCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCT744360.13019998385881226No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT728870.12749054521356937No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTC726660.12710398230808279No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT715000.12506446942212204No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC710630.12430008937824137No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA701300.12266812923878907No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCACTGTAGGCACCATCAAT692120.12106240640061414No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA672960.11771102845078496No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT660890.11559980027466607No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT660000.11544412562042035No Hit
CTCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATC657800.11505931186835228No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGCTGTAGGCACCATCAATATC654760.11452756922913095No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC633520.1108123673682556No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAA628340.10990630589747714No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT601360.10518708997438785No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAT596170.1042792793501909No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCCTGTAGGCACCATCAATATC586650.10261408529578725No Hit
CTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCT574210.1004381384431842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)