FastQCFastQC Report
Sat 1 Jul 2023
SRR2724717.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724717.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54740127
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC6024461.1005564528558731No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC4719990.8622541193592773No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT3779360.6904185662557926No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA3457670.631651804534542No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATC2648890.4839027867070897No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGC2572590.4699642001195941No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC2435680.44495329724024935No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2232800.4078909060623846No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCT2085280.3809417541175964No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1970860.360039354676689No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCA1938980.3542154734131326No Hit
TGGGCCAGCTTTAGCTCAGCGGTTACTTCGACCTGTAGGCACCATCAATA1874980.34252386736333296No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAT1817500.33202334367985664No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATAT1712670.3128728583329739No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT1602600.29276512274076383No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA1586340.28979472407873663No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAA1534060.28024414338680653No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1494320.2729843867552591Illumina PCR Primer Index 6 (96% over 29bp)
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1479130.27020945713187694No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAT1451190.2651053403657613No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1423910.2601217932870342No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTCG1403080.2563165408805135No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATC1284530.23465966748670497No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATATC1247910.2279698766500852No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA1085020.19821291243989989No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1081550.1975790081743873No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCAT1006770.18391809723057456No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCT982280.17944423110308091No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT949550.17346507069667558No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC910510.16633319100629781No Hit
CCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCA883660.16142819690571783No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCT883310.16136425843513297No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT844560.15428535633466836No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAT827310.15113410314155828No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAT819150.14964342337020883No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCTGTAGGCACCATCAATATCT791220.1445411334175385No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGC775030.14158352244962824No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA764290.1396215248093962No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGCTGTAGGCACCATCAAT755310.1379810463355337No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC706190.1290077386923125No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT697460.12741293055458203No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTC689930.1260373400302853No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA666140.12169135084396131No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT662250.12098072041374694No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCTGTAGGCACCATCA655980.11983530838355562No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATATC645260.11787696437021421No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATATCTC645150.1178568694223161No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATC644540.11774543380215395No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAAT643880.11762486411476539No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT587330.10729423408170026No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATC586880.10721202747666259No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC579500.10586383915404507No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCATC565080.10322957416594959No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCA548160.10013860581653382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)