FastQCFastQC Report
Sat 1 Jul 2023
SRR2724718.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724718.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53348273
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC6128921.1488506853820741No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCAC5561601.0425079739694667No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT3445980.645940310007786No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA3122550.585314167527035No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGC2747260.5149669980882043No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGCA2613920.4899727494458912No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2600350.48742908697344334No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC2035120.38147814082004117No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1985780.3722294815429171No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATAT1977710.37071678027890426No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCTCG1821870.3415049630566298No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATATC1798960.33721054100476694No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCT1776690.33303608534806745No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATC1661180.3113840254960081No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1547210.29002063478231055No Hit
TGGGCCAGCTTTAGCTCAGCGGTTACTTCGACCTGTAGGCACCATCAATA1452700.2723049722715485No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA1438650.2696713350027282No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1173090.2198927789096378Illumina PCR Primer Index 6 (96% over 29bp)
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAA1131090.21201998422704332No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT1119830.2099093254621382No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCT1078780.2022146058973643No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT1025660.19225739509880665No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAT998470.18716069777928895No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCAT925260.17343766685755693No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAT908110.17022294236216418No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG882970.16551051240215406No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT840650.15757773452197787No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGCTGTAGGC838470.15716909898845274No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT823460.15435551212688742No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAAT787120.14754367025151874No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAAT781240.1464414789959555No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAT779990.1462071696304021No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC779170.14605346268659905No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA767480.14386220149994358No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT751130.140797434998505No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGCTGTAGGCACCATCAATA723920.13569698872913843No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA721820.1353033489950087No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT716720.1343473667835508No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGTGGACTGTAGGCACCATCA713260.13369879845969895No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATATCT698340.1309020818724535No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG690390.12941187430753381No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT676630.12683259681152192No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATC676130.12673887306530054No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATC657260.12320173888290628No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATATCTC654760.12273312015179946No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATC646450.12117543148960043No Hit
CCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCA642700.1204725033929402No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATATC622100.11661108504862003No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA609280.11420800819550428No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA602340.11290712259795176No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC595090.11154812827774199No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATAT592770.11111325009527488No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATATCT584490.10956118485784909No Hit
TAGTGAGGCCCTCGGATCGGCCCCGCCGGGGCTGTAGGCACCATCAATAT566180.10612902127122277No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCTGTAGGCACCATCA555040.10404085620541081No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATATC552500.10356473957460628No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCTC542070.10160966222842865No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)