FastQCFastQC Report
Mon 25 Sept 2023
SRR2724719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36790185
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT20056315.451538229557693No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT19940875.42016029547011No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC7927392.1547567646099086No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC7283691.979791621053278No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC5562641.511990222392195No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA5483671.4905252582991904No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATT5246251.425991742090995No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT4928661.339667087838781No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA4321511.1746366592067965No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT4138431.124873386747036No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2912220.7915752530192496No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2730990.7423148320673028No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT2601160.7070255286838052No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2536870.6895507592582097No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTA2445590.6647397940510492No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT2184570.5937915234729045No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTAT2163570.5880834793301528No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1970310.5355531645192869No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1919720.5218022143677723No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC1901270.5167872898709261No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATTCG1852270.5034685202045056No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1585030.4308295813136031Illumina Single End Adapter 1 (95% over 22bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTA1463800.3978778579123753No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATT1385710.37665208805011446No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1242360.33768789148518824No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT1180510.32087634242665536No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTA1159920.3152797410505003No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1150510.3127219936512958No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATTCGT1127680.30651653423324726No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATTCGTA1117290.3036924114407144No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1080860.2937903139111695No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA1065860.28971313952348976No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT1065800.28969683082593906No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATTCG999820.27176269975266504No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTATG979130.2661389172139254No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT959240.260732583975862No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT859890.2337280989481298No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA842340.22895780491454445No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG781710.21247786603954288No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTATGC733650.19941459930141692No Hit
CCTCCCGGGGCTACGCCTGTCTGGGCGTCGCTCTGTAGGCACCATCAATT732140.19900416374639052No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATTC729510.19828929917041732No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGT728510.19801748754457202No Hit
TCTCCCGGGGCTACGCCTGTCTGGGCGTCGCTCTGTAGGCACCATCAATT724660.19697101278506754No Hit
TACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATTCGT720600.19586745758413557No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATTCGTA701180.1905888758102195No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTC694630.18880850966093268No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT693180.18841438280345696No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT690040.18756089429830264No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT671550.18253509733642273No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT666520.1811678848584208No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG644770.17525598199628514No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT642310.17458732539670568No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT640480.17408991012140873No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT636300.17295373752537532No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATT614390.1669983448031044No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATT603470.16403016184887356No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA600480.16321744508759606No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTATG594480.16158657533252416No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATTCGT590330.1604585570852661No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA588000.1598252359970465No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATCA581860.15815631261435625No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA579720.15757463573504726No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT572990.15574534349310828No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAAT557040.15140994806087546No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG542660.14750129688121982No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA540470.1469060294206186No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTA531820.14455485885705657No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTATG528270.1435899275853057No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA515870.14021946342482378No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT500490.1360390006193228No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT489380.1330191734561813No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT484430.131673705908247No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC468040.12721871336064225No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT464750.12632445311161117No Hit
CCCTACCTACTATCCAGCGAAACCACAGCCCTGTAGGCACCATCAATTCG459820.12498442179619373No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGCTCTGTAGGCACCATCAATT453870.1233671426224141No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA450240.12238046642059561No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATT433370.11779500429258512No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA431630.11732205206361425No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC408330.11098884118141836No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA401060.1090127706615229No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGT394960.10735471974386646No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT392580.10670780807435461No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATT382850.10406308095487968No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTAT376810.10242133873477395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)