FastQCFastQC Report
Mon 25 Sept 2023
SRR2724720.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724720.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40702194
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA21977325.3995418527070065No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA12997903.193415077329738No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA8035551.9742301852327666No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC6609141.6237797893646717No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG5582091.3714469544319896No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA4871231.1967978925165557No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT4617621.1344892120557433No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTC4510171.108090143740163No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT4395641.0799516114536725No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT4157561.0214584501267916No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAT2587980.635833046248072No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC2450340.602016687355969No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT2049740.5035944745386453No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1970050.4840156773858431No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1890630.46450321572345704No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCG1874270.46048377637824633No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC1690080.41523068756440995No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT1676560.4119089993035756No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1659470.40771020844723993No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA1646690.40457032856754604No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTCGT1628160.40001774842899135No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1623410.39885073517167163No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1578190.38774076896198767Illumina PCR Primer Index 6 (96% over 29bp)
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1454620.3573812261815665No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT1391790.3419447118747456No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCGT1350730.33185680359147224No Hit
GGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT1276140.3135310101465292No Hit
CTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1193030.29311196344845686No Hit
AGGTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATATCT1168240.2870213826802555No Hit
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATAT1159420.28485442332666394No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCTC1147710.28197742853861885No Hit
TACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCT1106460.27184283972505263No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA1065560.26179424136202584No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1051850.2584258725708988No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCGTA1000840.24589337862229244No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT999390.24553713247005798No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT987580.24263556898185884No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT983470.2416257954055253No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAAT960680.236026588640406No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA955370.23472199066222327No Hit
GTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA908720.22326069204033572No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT880290.21627581058652512No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCTC863750.21221214758103704No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATA857480.21067169008137498No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC848170.2083843440970283No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA832520.20453934252291167No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTC821050.2017213126152364No Hit
ATGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA739550.18169782198964507No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT674270.16565937452904875No Hit
GACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC668000.16411891702938666No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT654880.16089550356916876No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT650950.15992995365311266No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCCTGTAGGCACCATCAAT642040.15774088246938237No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATATCTC640670.15740429127727118No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT637300.15657632608207803No Hit
TGGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATA620450.1524365001061122No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATATC617620.1517412058917512No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCT607580.14927450839627956No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT606430.14899196834450742No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATATCTCG587010.14422072677458123No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATA585060.14374163712157628No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT582660.1431519883178779No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATAT571030.1402946484899561No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATATCTCG569940.14002684965827641No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT569530.1399261179876446No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCCTGTAGGCACCATCA544660.13381588225931998No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA544400.13375200363891931No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATC541630.13307145064465076No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAAT539310.13250145680107564No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT538550.13231473467990448No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT534500.13131970232366344No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA531590.13060475314917913No Hit
GCGGCGTGGGAAATGTGGCGTACGGAACTGTAGGCACCATCAATATCTCG515750.12671307104476973No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATATCT508080.12482865174295026No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT507500.1246861532820565No Hit
ACGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCTGTAGGCACCATCA478630.11759316954756789No Hit
GACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT466240.1145491075984749No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG448540.11020044767119926No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCTGTAGGCACCATCAATA444680.10925209584525099No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATATC438520.10773866391575845No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTCTGTAGGCACCATCAATATCTCGT436150.10715638572210628No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT432240.10619574954608098No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTC431110.10591812323433965No Hit
AGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCTC422010.10368237152031656No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATGCTGTAGGCACCATCAAT416950.10243919529251912No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATAT415390.10205592357011516No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATAT408740.10042210500986752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)