FastQCFastQC Report
Tue 26 Sept 2023
SRR2724721.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724721.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29893959
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT9587233.207079396877476No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA9053973.02869553009021No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT7600912.5426240800022506No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC5975091.9987616896109344No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG5817241.9459583790825432No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT3647791.2202431936164762No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTC2796190.9353695842026144No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT2795270.9350618297161644No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT2622180.8771604992165808No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG2012710.6732831874158923No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA1725640.5772537521711326No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCGTA1640330.5487162138678252No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTCGT1562610.5227176500777297No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT1484740.496668908925713No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCG1464640.4899451424282745No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCTCGT1456350.48717200689276385No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC1386400.4637726304501856No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCGT1220060.4081292812370553No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATATC1163230.3891187513838498No Hit
AGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATATCTCG1067840.357209294359439No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC1028780.34414310931516295No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT1024370.3426678948746802No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAT1023050.3422263340897738No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGT1012470.3386871574955997No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA958520.32064003299128097No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATATCTCG948210.31719117564856497No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCTC913350.30552995673808214No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA875500.29286853574663696No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTC865380.28948323639568785No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCG846770.28325789835999976No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT840530.28117052010407856No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA801470.2681043350598026No Hit
TCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTCGT793300.2653713414138288No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATC792230.2650134095654577No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC788320.2637054529980455No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTC787590.26346125650336244No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATATCTCG771920.25821939476132955No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT718950.24050009568822917No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA711240.23792097928548037No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATAT687150.2298624949609384No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCT685190.22920684409850162No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAAAATCT682230.22821667748992364No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA678410.2269388273396642No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATATCTCGT674260.22555058699317812No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATAT667560.22330933149403198No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTCTGTAGGCACCATCAATATCTCGT664820.222392758349605No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA660290.2208774020195853No Hit
TGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCCTGTAGGCACCATCA656380.21956944545217313No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATATCTC654170.21883016565320104No Hit
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATAT611300.20448947561612701No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT603870.20200402362229772No Hit
AGGTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATATCT595230.1991138075756376No Hit
TGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACCTGTAGGCACCATCAA567640.1898845181396014No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATC564850.1889512192078674No Hit
AGTACGAATACAGACCGTGAAAGCGGGGCCTCCTGTAGGCACCATCAATA553760.18524143958316128No Hit
GACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC551910.18462258545280003No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT546690.18287641325794285No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATATC534160.17868493095879337No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATATCT516520.1727840731968623No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAATA498430.16673268334916763No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATATCTCGT489430.16372204163389667No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATATCT485390.16237059801948614No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT476410.15936664661913802No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT453020.15154232331689488No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATATCT449930.1505086696613185No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA447110.14956533525720028Illumina PCR Primer Index 6 (96% over 29bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATATCTC441700.1477556050705763No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTGTCTGTAGGCACCATCAATATC432690.14474161819784392No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT426250.14258733679269447No Hit
ACGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCTGTAGGCACCATCA420290.1405936229456928No Hit
GCGGCGTGGGAAATGTGGCGTACGGAACTGTAGGCACCATCAATATCTCG408060.1365024953703857No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAT407270.13623822793093412No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA406710.13605089911309506No Hit
TGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATA400590.1340036627467108No Hit
TCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCG399380.13359889869387992No Hit
TGAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAATA397970.13312723149182082No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTCG392090.13116027890451046No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATC391980.1311234821724349No Hit
TGGACCAAGGAGTCTAACGCGTGCGCGAGTCCTGTAGGCACCATCAATAT391710.13103316292097678No Hit
TACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCT388000.12979210950279285No Hit
TTCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCA387940.12977203855802438No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA382300.1278853697497879No Hit
TTGACGTACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATATCTCG369620.12364371008871725No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA367720.12300813017104893No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATA365330.12220863753777142No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATATCTCG362420.12123519671650047No Hit
TGGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATA362080.12112146136281246No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA359230.12016809148630998No Hit
TGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCA359060.120111223809466No Hit
CTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT357980.11974994680363346No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCG355490.11891700259574184No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT352540.11793018114462524No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT349110.11678279213536086No Hit
AAGGAGTCTAACGCGTGCGCGAGTCCTGTAGGCACCATCAATATCTCGTA347490.11624087662661209No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT344340.11518715202626724No Hit
CCCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC340960.11405648880430994No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAACTGTAGGCACCATCAATATCTC339510.11357144097240517No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCTC327820.1096609519000143No Hit
ACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTCGT325560.10890494631373515No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT324250.10846673068629017No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA323350.10816566651476306No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATATCTC317130.10608497857376468No Hit
GAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAATAT316890.10600469479469078No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCTGTAGGCACCATCAATATC313460.1048573057854264No Hit
GAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAA311950.10435218700875316No Hit
TATCCAGCGAAACCACAGCCAAGGGAACCTGTAGGCACCATCAATATCTC310260.10378685539777452No Hit
GGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAT310080.10372664256346908No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAACTGTAGGCACCATCAATATC305230.10210424119468418No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT303800.10162588367770224No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGACTGTAGGCACCATCAATATC302270.10111407458610618No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)