FastQCFastQC Report
Mon 25 Sept 2023
SRR2724723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42148183
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA11996132.8461796324648203No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA10989632.60737930268548No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC7277201.7265750222257503No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA7023781.6664490613984475No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG6527131.5486148003106088No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT6160021.4615149602059951No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT5647181.339839489640633No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT5598311.3282446837625241No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT5276211.2518238330700993No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT4933301.1704656402388687No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTC3632130.8617524508707765No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT3617700.8583288157404081No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3603040.8548506112351273No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC2553460.6058292002765576No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG2317050.5497390006112481No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT2017100.4785734179810313No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1962330.46557878900734584No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT1820520.4319332105016247No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT1814730.43055948580274506No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT1771820.4203787385093208No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC1702650.40396759214982053No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA1673860.39713692995970906No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT1582960.3755701639617537No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATC1517180.3599633227368307No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1514550.3593393337976159No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCG1484830.3522880215263372No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1431390.339608945894536No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1388130.32934515824798427No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC1367130.3243627370603378No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA1352780.3209580825821127No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT1324530.3142555397939693No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTCGT1283720.3045730346193097No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCTCGT1252450.2971539722127523No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA1248120.29612664441549No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA1218120.28900889986170936No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCGT1121190.2660114672084441No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATATCTC1100010.26098633955347494No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1085310.2574986447221224No Hit
AGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATATCTCG1071820.2542980322544391No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCGTA1070310.25393977244523214No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTC1070150.25390181114094523No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC1052140.24962879182715897No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTCTGTAGGCACCATCAATATCTCGT1031720.24478398036755228No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT1010260.23969242043008118No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA985330.23377757470588947No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT975940.23154972066055612No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC960200.22781527735133922No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC955720.22675236083130795No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT954220.22639647360361892No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATATC934950.22182450901857384No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT892210.2116840956109543No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTC860600.20418436543278745No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATATCTCG846720.20089122228590497No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATATCTCG840470.19940835883720062No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA837230.19863964242539234No Hit
AGGTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATATCT834460.19798243734492657No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAT811470.1925278724352127No Hit
TGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCCTGTAGGCACCATCA794590.18852295483295212No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA791680.1878325336112354No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT790960.18766170774194466No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAT790090.18745529314988502No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATC752840.17861742699560737No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGT749290.17777516055674333No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTGTCTGTAGGCACCATCAATATC748980.1777016105296876No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCTC733680.17407156080725947No Hit
ACGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCTGTAGGCACCATCA710760.16863360396817106No Hit
GACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC708120.16800724244743837No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATAT705560.1673998615788491No Hit
AGTACGAATACAGACCGTGAAAGCGGGGCCTCCTGTAGGCACCATCAATA685330.162600129168083No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA683760.1622276338697685No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATAT673780.15985979751487744No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCG673510.15979573781389345No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATATCT664550.15766990477383094No Hit
TGAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAATA653110.1549556715173226No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT637890.15134460244703787No Hit
CTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT629610.1493801049501944No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT572600.13585401771649325No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCTGTAGGCACCATCAATATC572110.13573776122211484No Hit
TTCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCA567150.1345609607892231No Hit
TGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACCTGTAGGCACCATCAA563350.13365937981241088No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT554520.13156439033208148No Hit
TCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTCGT546960.12977071870452875No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT536750.1273483129747254No Hit
TACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCT535810.12712529031204026No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATATCTCG532180.1262640432210328No Hit
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATAT530940.12596984311280987No Hit
TATCCAGCGAAACCACAGCCAAGGGAACCTGTAGGCACCATCAATATCTC523970.12431615379481482No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATATCTCGT523280.12415244567007787No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA522720.12401958110507397No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA520770.12355692770907824No Hit
GAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAATAT520470.12348575026354042No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATATCTCGT519180.12317968724772786No Hit
ACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTCGT518210.12294954684048895No Hit
TGGACCAAGGAGTCTAACGCGTGCGCGAGTCCTGTAGGCACCATCAATAT504340.11965877627512438No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA498930.11837520967392591No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATATC491190.11653883157905051No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT480900.11409744519710376No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT479020.1136513998717335No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA476080.11295386090546299No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATATCT476000.11293488025331958No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA469210.11132389740264723Illumina PCR Primer Index 6 (96% over 29bp)
ACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTGTAGGCACCATCAATA468330.11111511022906967No Hit
GAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAA465040.11033453090967171No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT462360.10969867906286732No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCT460820.10933330150910657No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT454730.1078883993646891No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATC450110.10679226670340689No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAA449040.1065384004809887No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA447090.10607574708499297No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATATCT440900.10460711912539623No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC437620.10382891238751621No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT436020.1034492993446479No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCTC434370.10305782339418998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)