FastQCFastQC Report
Mon 25 Sept 2023
SRR2724724.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724724.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46447212
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG16676923.590510448721874No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT12763652.747990557538739No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7139141.5370438165373628Illumina PCR Primer Index 6 (96% over 29bp)
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA6251061.345841812851975No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT5264901.133523364114944No Hit
AGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATATCTCG3761540.8098526990166814No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCTCGT3729390.8029308626748145No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3655430.7870074096158882No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3264210.7027784574023518No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGT3231040.6956370169214893No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA3041420.6548121768858807No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCG2765040.5953080671451281No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT2700380.5813868871182193No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTCTGTAGGCACCATCAATATCTCGT2336670.5030807877122958No Hit
TTGACGTACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATATCTCG2307730.4968500585137381No Hit
ACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTCGT2122750.4570242020123834No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2107220.4536806213470897No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTC1994990.42951770711232357No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA1978620.4259932759796217No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1878000.4043299735622452No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT1855950.3995826487927844No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1855360.3994556228692478No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT1837570.39562546832735623No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1830980.39420665335090516No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATATCTCGT1808200.38930216091333963No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC1769460.3809615095950215No Hit
AAGTTCAATTTTAAACTTGCTAAAAACTGTAGGCACCATCAATATCTCGT1741410.3749223957726462No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1724730.37133122220554377No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATATCTC1617830.34831584724611675No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATC1479020.3184303075069393No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1442360.31053747639363155No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATATCTCG1401880.30182220624996825No Hit
TTCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCA1380370.2971911424952697No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG1344530.28947485588586025No Hit
TAACCTAGACTTACAAGTCAAAGTAACTGTAGGCACCATCAATATCTCGT1261460.27159003644825874No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATATCTCG1137030.24480048447256642No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1106090.2381391589230372No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA1096700.23611750905522597No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1089450.23455659728295425No Hit
TTGACGTACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATATCTCGT1063350.2289373149027761No Hit
TCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCG1034840.22279916391967722No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT1033970.22261185450700463No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT937580.20185926337193283No Hit
GGAATGCCTAAAGGAAAGATCCAAAAAGATCTGTAGGCACCATCAATATC930890.20041891857793315No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCAAT926120.1993919462808661No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCG904990.19484269583285213No Hit
TGAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAATA891950.19203520762451792No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCTGTAGGCACCATCAATATC872710.187892870728172No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATATCTC866550.1865666339671798No Hit
AAGTTCAATTTTAAACTTGCTAAAAAACTGTAGGCACCATCAATATCTCG840790.18102055296666675No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT840130.18087845617084616No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA840040.18085907933505244No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT830840.17887833612058351No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC822580.17709997319107118No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC816040.17569192312339438No Hit
CTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT815400.1755541322910835No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCAA808170.17399752648232147No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCAATA804640.17323752392285677No Hit
TATCCAGCGAAACCACAGCCAAGGGAACCTGTAGGCACCATCAATATCTC804450.1731966172695145No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA800420.1723289656223069No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATATCT776460.16717042133766824No Hit
ATGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT771850.16617789674867892No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG750260.1615296091399415No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATATCTCGT730930.15736789540780186No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC723330.15573162927411016No Hit
TAACCTAGACTTACAAGTCAAAGTAAACTGTAGGCACCATCAATATCTCG709490.1527519025253873No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATATCTCGTA706650.15214045570700777No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT695080.1496494558166376No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC681690.14676661324688337No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT680470.14650394947279075No Hit
ACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCAATATCTCGT677060.14576978269438431No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA674410.14519924252934707No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA670310.14431652000985548No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCCTGTAGGCACCATCAAT658690.14181475521071102No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCCTGTAGGCACCATCAATAT652070.14038948128899534No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT640290.13785326878177318No Hit
TTCCATCTAAGGCTAAATACCGGCACGAGACCTGTAGGCACCATCAATAT637470.13724612792690333No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT632770.1362342265021203No Hit
AGTACGAATACAGACCGTGAAAGCGGGGCCTCCTGTAGGCACCATCAATA626710.13492951955867663No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATATCTCG615360.13248588526691335No Hit
GAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAATAT611610.13167851710884176No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCG598480.1288516520647138No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC592420.12754694512127016No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT572860.1233357128087688No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCCTGTAGGCACCATCAA565370.12172312947438052No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCGTA561300.12084686590015349No Hit
TCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTCGT553080.1190771148976606No Hit
AAATAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCA551640.11876708552496112No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC545180.11737625931132313No Hit
TAACCTAGACTTACAAGTCAAAGTAAAATCCTGTAGGCACCATCAATATC543520.11701886434001679No Hit
TGGAATGCCTAAAGGAAAGATCCAAAAAGATCTGTAGGCACCATCAATAT537870.11580242964852228No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATATCTCG537280.11567540372498569No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT534100.114990755526941No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATA532330.1146096777563312No Hit
CGGAATGCCTAAAGGAAAGATCCAAAAAGATCTGTAGGCACCATCAATAT531670.11446758096051061No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT530000.1141080330074494No Hit
GACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTCG528610.1138087685435242No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT522650.11252559141762912No Hit
AAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCAATA520890.11214666662877418No Hit
GTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT517690.11145771246721978No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATC509090.10960614815804229No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT508610.10950280503380914No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAACCTGTAGGCACCATCAATATCT506140.10897101854035933No Hit
ACCTCTAGCATTACAAGTATTAGAGGCTGTAGGCACCATCAATATCTCGT483040.1039976306866384No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT474960.10225802142871353No Hit
TTAACCTAGACTTACAAGTCAAAGTAACTGTAGGCACCATCAATATCTCG468270.10081767663471383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)