FastQCFastQC Report
Mon 25 Sept 2023
SRR2724725.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724725.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71098080
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT22164823.117499094209014No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT18228532.563856858019232No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA17696092.488968759775229No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC15557082.1881153471373627No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC10997881.546860337156784No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA9473071.3323946300659595No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT8401721.1817084230685273No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC7377841.0376989083249506No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG7020530.9874429801761172No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTAT5967040.8392687960068682No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT5753490.809232823164845No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA5727950.8056406023903881No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATT5505660.7743753417813815No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5293860.7445855077943033Illumina Single End Adapter 1 (95% over 22bp)
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATT5276920.742202883678434No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC4607410.6480357838073827No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT4474820.6293868976489942No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATCA4349110.6117056888174758No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT4265640.5999655686904626No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3816590.5368063385115323No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3611010.5078913523403163No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA3368820.4738271413236476No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATT3165080.4451709525770597No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA3007910.4230648703874985No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2831850.398301895072272No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTA2722130.3828696921210812No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTC2683310.3774096290645261No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTA2657810.3738230343210393No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC2640470.37138414989546836No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT2634720.37057540794350563No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTA2608260.36685378845673466No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT2560960.3602010068345024No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA2436960.342760310826959No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC2419750.3403397110020411No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTA2110600.2968575241412989No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2082960.29296993674090777No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCAT2018890.2839584416344295No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATTCGTA1959170.2755587773959578No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATT1936750.2724053870371746No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1927190.2710607656353026No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCTGTAGGCACCATC1888520.2656218001948857No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1873220.26346984334879364No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1857650.2612799107936529No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1856490.26111675589551786No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATTCGT1767520.24860305651010547No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA1711610.24073927172154297No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATTCGTA1702510.23945934967582808No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT1596360.22452926998872544No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATTC1585800.22304399781259915No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATTCGT1554820.21868663682619838No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1530170.2152195952408279No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA1523790.2143222433010849No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATTCG1482470.20851055330889387No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTATGC1472190.2070646633495588No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCTGTAGGCACCAT1471820.20701262256308467No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTATG1407350.19794486714690465No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC1374600.19333855429007366No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATTCGT1359390.19119925601366453No Hit
GACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTAT1346190.18934266579350667No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTCTGTAGGCACCATC1345640.18926530786766674No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1286560.18095566012471784No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATTCGT1273330.17909485038133238No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA1234850.17368260858802376No Hit
CCCTACCTACTATCCAGCGAAACCACAGCCCTGTAGGCACCATCAATTCG1224300.17219874291964005No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG1192890.16778090210030988No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGCTGTAGGCACCATCAATT1184090.16654317528687132No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAA1136420.15983835287816492No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT1106710.15565961837506723No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTATG1105900.15554569124792117No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGT1075620.1512867858034985No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT1025800.14427956423014518No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATT1018370.14323452897743513No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1000460.14071547361053913No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGT991660.13947774679710057No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAAT986680.13877730594131374No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT943830.13275042026451347No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATTCGTAT941900.1324789642702025No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATT918420.12917648409070961No Hit
TACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATTCGT913160.1284366610181316No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTATG884370.12438732522734791No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT880720.12387394990131942No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATTCGTATG873270.12282610163312428No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCA867370.12199626206502341No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATTCGT844570.1187894244120235No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATTC835980.11758123426117836No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGTAGGCACCATCAAT833630.11725070494168056No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT829100.11661355693430821No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCATC827790.11642930442003498No Hit
TTCCCTACCTACTATCCAGCGAAACCACAGCCCTGTAGGCACCATCAATT821290.1155150743873815No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATT810600.11401151761060214No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATTCG774900.10899028496972069No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC753020.10591284602903482No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA744080.10465542810720065No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAAT742470.10442898036065108No Hit
TTCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCA741530.10429676863285198No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA736750.10362445793191603No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT732950.10308998498974938No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATTC730840.10279321185607262No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT730200.10270319536054982No Hit
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATTC727650.10234453588620115No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAATTCGT725970.10210824258545378No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCCTGTAGGCACCATCA722140.1015695501200595No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG717130.10086488974104504No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)