FastQCFastQC Report
Mon 25 Sept 2023
SRR2724727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71079984
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTAT15185732.136428449393011No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT14264932.0068842446559922No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC12378061.7414269536132703No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA11909831.6755532753074338No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA8437141.1869923887433627No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT7927461.115287251612212No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7506751.0560989996846368Illumina Single End Adapter 1 (95% over 22bp)
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA7395831.0404940440054122No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT7287091.025195785075022No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTA5884520.8278730057114251No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTA4807810.6763943559694667No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT4717540.6636945782092466No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC4629310.6512818010763761No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC4361220.6135651352988487No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC4323770.6082964228016708No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATT3868120.5441925816978237No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATCA3588300.5048256623130359No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT3504510.49303753360439695No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG3383930.4760735455427227No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATT3304280.46486785928370494No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT3225420.4537733154244942No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA3195070.44950347765975857No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTA3144170.4423425306342219No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATT2999780.4220287950543152No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT2977910.41895197950522894No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATT2966430.41733689754347725No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCA2893610.40709210063975254No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC2838690.3993655935544386No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTATG2837190.3991545636813874No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA2809740.3952927170045508No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC2768750.3895259740069722No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2717160.38226795323983187No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2653810.37335545826797034No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATTC2630830.37012248061282627No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATTCGT2528050.3556627137113593No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTC2497550.3513717729593186No Hit
GACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTAT2432230.3421821254208498No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG2364520.3326562369513195No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT2310470.32505212719237525No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT2292020.32245645975384574No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC2149940.3024677101784379No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCG2101480.29565003841306436No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATTCGTA2077130.2922243201405335No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2056870.2893740099885222No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT2035510.28636894459627343No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG1966360.2766404674486139No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTATGC1946810.2738900447698469No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1920730.270220938710397No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGT1751270.24638019051889487No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATTCG1664030.23410669310223817No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC1657670.23321192644050118No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA1573670.221394253549635No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATTCGT1566000.2203151874654333No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATTC1535110.2159693789463993No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAA1526000.21468772418406848No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCGT1508480.21222289526683066No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCG1416510.1992839503171526No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGCTGTAGGCACCATCAATT1394000.19611709535556449No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGT1390920.1956837806828994No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC1343260.18897865818315324No Hit
GAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAA1316290.18518434106569298No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCTGTAGGCACCATC1297380.18252395779942776No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATTC1275930.17950623061479587No Hit
CCCTACCTACTATCCAGCGAAACCACAGCCCTGTAGGCACCATCAATTCG1273540.17916998968373432No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTAT1232130.17334415832170136No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG1215070.17094404523219925No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC1197970.1685383046794158No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAA1187960.16713003199325424No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG1149420.16170797112165924No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCCTGTAGGCACCATCAATTCGTAT1149270.16168686813435412No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT1134350.15958782433040503No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1124810.1582456743377995No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCTGTAGGCACCATCA1109580.15610301769341986No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT1109430.15608191470611474No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT1086930.152916466610347No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATTCGTAT1065520.1499043668889965No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA1029280.14480588515607995No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATTCGTAT1026600.1444288451162285No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTATG1020830.14361708353789163No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1000860.1408075724946702No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC996690.14022090944758794No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATTCGT976450.13737341302721734No Hit
TGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCA975930.13730025600455958No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT975460.13723413331100356No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT969810.1364392541225108No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT958100.1347918142468912No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATTCGTA937340.13187116080386288No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA933280.13129997328080434No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGTAGGCACCATCAAT880150.12382529517733148No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATTCG865500.12176423675053162No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATTCG860230.1210228184632118No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAA825590.11614943526154986No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCTGTAGGCACCAT811890.11422202908768242No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATTCG811800.11420936729529933No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT800590.11263227071069684No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATTC799480.11247610860463896No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTCG763130.10736215134769866No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTA752870.10591870701602858No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA749750.10547976488008214No Hit
TGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCCTGTAGGCACCATCA730160.1027237147380337No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATTC723390.1017712665776627No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC717020.10087509305010535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)