FastQCFastQC Report
Mon 25 Sept 2023
SRR2724729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78540591
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT15957342.031731592139407No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTAT14321301.8234265642335186No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT12206621.5541797998438796No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG11620211.479516496126188No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC11540731.469396888037168No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTA7653250.9744324434737193No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA7333900.9337719396585645No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT7266100.9251394606898234No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC7211020.9181265264479611No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6750660.8595122488956062Illumina Single End Adapter 1 (95% over 22bp)
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA6541910.8329336355515837No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT6536220.8322091693962426No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC5882290.7489490370654328No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG5880960.748779697876223No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCGT5809060.7396251958430005No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCG5697630.7254376275319854No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA5276180.6717774761842573No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT5146430.655257356033901No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATT4925360.6271101270424614No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATT4280900.5450557406679051No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTA3954770.5035319889558764No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT3889150.4951770734702009No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT3731420.47509446421150564No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA3518310.44796072390135183No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC3429220.4366175446782671No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATT3333730.4244595001837967No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT3156430.40188518571244264No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCA2808220.35755014881413355No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2741320.349032260274181No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATTCGT2631770.33508405863663543No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC2629690.3348192274234351No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA2504080.31882622324550625No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCG2424990.30875627100896147No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTATG2340180.2979580329361158No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATT2309110.29400211668893605No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA2294030.29208209039323374No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTATGC2269920.2890123401286858No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2267990.2887666073202836No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC2218950.2825227021782915No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT2212880.2817498533974617No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2044960.2603698258394822No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGT2000020.2546479437619714No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCTGTAGGCACCAT1969080.2507085794656167No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT1954190.24881274448265867No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATTCGT1933670.2462000827062786No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCGT1928060.24548580236682963No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAA1888060.2403928944206697No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC1860170.23684186435520962No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG1784590.2272188147909404No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGCTGTAGGCACCATCAA1725000.2196316551781486No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATTCGT1716100.21849848316012802No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC1707680.21742642603746135No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT1631570.20773589544290544No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATTCG1583120.2015671106931192No Hit
GGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCGT1567930.19963307890056492No Hit
GAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAA1565380.19930840601899724No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGCTGTAGGCACCATCAATT1553190.197756342322405No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1506030.19175180385388238No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC1483190.18884375341662504No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATTC1468390.18695937747654584No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG1436680.18292197470222754No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTAT1395250.17764699529699235No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAA1315550.16749937621426864No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1302740.1658683724445109No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1290520.164312489066959No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATTC1279220.16287374257216883No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCAAT1273970.16220529840423534No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATTC1251910.15939655967192812No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATTCG1233400.15703981651984258No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATTC1232560.15693286545297322No Hit
GGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCGTA1211700.1542769139590508No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATTCG1201220.1529425720771569No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTA1200830.15289291622468182No Hit
CCCTACCTACTATCCAGCGAAACCACAGCCCTGTAGGCACCATCAATTCG1171180.14911779820959076No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1168810.1488160434137808No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATTC1158910.1475555486971062No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT1155700.14714684283442686No Hit
TGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCA1129170.14376897163913624No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1123820.14308779520133735No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC1114510.14190242087686863No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT1076320.13703996701527238No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTC1072040.1364950258650333No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1069800.13620982302004833No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATTCGTAT1059750.13493022989857562No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATTCGTA1038220.132188972196555No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCTGTAGGCACCATCA1002920.12769448093406885No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA995150.1267051835655273No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATTCGTA992290.12634104064737683No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT985680.1254994376092739No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGTAGGCACCATCAAT984490.12534792359787567No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTA975910.12425549484342434No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT975360.12418546735916464No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTATG973570.12395755972857399No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGT969070.12338460758463098No Hit
TGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACCTGTAGGCACCATCAA952060.12121884848052646No Hit
TTCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCA932460.11872332358690808No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATTCGT906410.11540656728697138No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATTC898530.11440326442157787No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATT891810.11354765588662301No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATCA885740.11277480710579324No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT883450.11248323812587557No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGT883080.1124361287273736No Hit
GGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATTCGTA877200.11168747125928809No Hit
AGTACGAATACAGACCGTGAAAGCGGGGCCTCCTGTAGGCACCATCAATT870940.11089043116571404No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAAT856730.10908117561784071No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC855200.10888637188890009No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC848090.10798110750147016No Hit
CGGAATGCCTAAAGGAAAGATCCAAAAAGATCTGTAGGCACCATCAATTC844590.10753547805618117No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCG841080.10708857538390562No Hit
GAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAATTC823000.10478658099224132No Hit
ACGAGCGTACGCGTTAGGACCCGAAAGATGGTCTGTAGGCACCATCAATT821840.10463888666180268No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCAA808930.10299515062217955No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATTCGT789300.10049580604760155No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)