FastQCFastQC Report
Mon 25 Sept 2023
SRR2724730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47939225
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA528965711.034089516465901No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA492059810.2642418603972No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT38502158.031450237253521No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA22048074.599171138039883No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT17637683.679175038812163No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT15691513.2732089431983935No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA14921903.1126702611483603No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA14460143.016348303503029No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT12755522.660768921483399No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA11198452.335968092934335No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA10706382.233323546636392No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT10092642.1052989488253093No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA8594031.7926927270935231No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA7808111.6287518206646021No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA7456901.5554903109092815No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT5288571.1031822062204801No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC5124531.0689638808303639No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG4993781.0416897644882661No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTC4776100.9962822719808258No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT4591170.9577063458994175No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT4082820.8516658331460302No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTC4066620.8482865544864358No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC3769270.7862601032870264No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA3189780.6653799680741606No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3057280.6377408061978473No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT2950640.615495974329998No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAT2936030.6124483656129193No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT2906430.606273881148475No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2774740.5788036832051415No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG2479780.5172757799067464No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2294020.478526717943396No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG2047430.4270886732107163No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1697390.3540712224696999No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1522670.3176250763336287No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1520840.3172433430035633No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC1509500.3148778479418472No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATCA1406970.29349035158578385No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1268240.26455162760766365No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC1228820.25632871620265035No Hit
CTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1210140.2524321158717105No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCG1205030.25136618291180135No Hit
GTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1200000.2503169377477421No Hit
GGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT1170280.24411742158952301No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCGT1132550.2362470398718377No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT1053700.21979913108732982No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT972150.20278800919288953No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT912700.19038689090197014No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATA877170.18297542357015575No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA871280.1817467846007106No Hit
TACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCT845500.1763691423880966No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC824060.17189681310033694No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT805000.1679209457391103No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCTC788190.16441442263616068No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT774490.16155663759687397No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA770630.16075145144711872No Hit
ATGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA740880.15454567736545594No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT738870.15412639649472848No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT722460.1507033123710281No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT715340.1492180985403915No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT710190.14814382168255744No Hit
GTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA682160.14229683521166644No Hit
TTTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA678750.1415855179135666No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC664560.13862552012469956No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT644290.13439724985124393No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA636330.13273681416418392No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATGCTGTAGGCACCATCAAT595150.12414677125047391No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA533810.11135140378260183No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA519620.10839140599373477No Hit
GACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC517470.10794292148027006No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCTC514040.10722743223320777No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA502700.10486193717149161No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA495260.10330997215745562No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT487290.10164745049591435No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCGT482840.10071919185176649No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)