FastQCFastQC Report
Mon 25 Sept 2023
SRR2724731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50638677
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT8175291.6144359379689166No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTAT7986321.5771186123207759No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT6561441.2957368534726925No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5394081.0652095037948957Illumina Single End Adapter 1 (95% over 22bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA4989100.9852350605447294No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATT4938110.9751656821523991No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT4555980.8997036000762817No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT4343750.8577929474737265No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC3938280.7777217402421474No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT3834940.7573144140396875No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC3433600.6780587889371597No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA3236460.6391280720070944No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTA3044940.6013071787005809No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCG3000830.5925964455982924No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2773920.547786823103613No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTC2652290.5237676331867833No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2646420.5226084401849599No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT2623260.5180348609818538No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATT2527890.4992014305587012No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA2511940.496051664224956No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC2431580.48018237127324637No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT2410990.4761163092787752No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATT2331990.46051558574486456No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG2174110.4293378359786137No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC2092960.413312535791565No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTA1981570.391315515608751No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1845120.36436970894796483No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATCA1744020.34440473237482094No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCGT1708590.33740810408613164No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTATG1632430.3223682166893894No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG1550110.3061118678120283No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC1544390.304982296437168No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTATGC1541890.30448860265444927No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATTC1538200.3037599106311565No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATTCGTA1517670.2997056972874706No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1459890.2882954465812762No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATTCGTAT1434200.2832222492700589No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC1418240.2800705081611828No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA1411480.2787355601727115No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC1389950.2744838693159381No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATTCGT1338170.26425848368826854No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC1335900.26381020973356No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG1325670.26179001477467506No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA1285750.25390671245222307No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCTGTAGGCACCAT1212710.23948295489631374No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1210130.23897346291254806No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA1184630.23393778632881743No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1159690.22901269715241576No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1121620.2214947282291755No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATTCG1088400.2149345252444095No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1063310.20997981444104474No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT1054570.20825386097666018No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1045180.2063995471287688No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1025990.2026099536526201No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATTCG966940.19094890650480462No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATTCGTA905930.17890080343133768No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATTCGT897460.17722816889548673No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATT869510.1717086724046918No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTCTGTAGGCACCATC836840.16525708205212392No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTA831960.16429339178825703No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATTCGTA817050.16134900206812275No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCA806760.15931695845845262No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATT798020.15759100499406808No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATTCGT797650.1575179383142257No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA779570.15394754487760412No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT774570.15296015731216675No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCTGTAGGCACCATC766020.15127172457526883No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG758780.1498419873805155No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA752390.14858010607188651No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA751320.14836880513288292No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT750100.1481278825669162No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAA749670.14804296723628857No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA748840.147879060900426No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT740220.14617680473761194No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG729560.14407169444809942No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTAT708870.13998588470231954No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGT701110.13845345920076071No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG699870.13820858708453224No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG681040.13449008551309508No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAAT671780.13266144374190503No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT669400.13219144726075682No Hit
GACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTAT662110.13075183619034914No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGCTGTAGGCACCATCAATT657190.12978024682595873No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATTCGTA656030.12955117291077728No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATTC655830.12951167740815978No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT641220.12662653094195175No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATTCGTAT641080.1265988840901195No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC635890.1255739757971955No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATTCGT617910.12202333011188266No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATTC595510.11759983381872319No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATTCG579650.11446784046115581No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATTC574050.11336196638786593No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATTCGTATG557700.11013320904888568No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATTCGT555240.10964741436669051No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT550170.108646203375337No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA549390.10849217091512876No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT538090.10626067501724028No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTA531460.10495139910547031No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC525580.10379023132851596No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCG525400.10375468537616021No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATTC523200.10332023484736777No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGT521140.10291343117040755No Hit
CCCTACCTACTATCCAGCGAAACCACAGCCCTGTAGGCACCATCAATTCG509170.10054962533875046No Hit
GGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCGT507580.10023563609294138No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)