FastQCFastQC Report
Mon 25 Sept 2023
SRR2724732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34486372
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT11820463.427574231351445No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA9845202.854808850290196No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG5855831.698012768638No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC5281821.5315673101247067No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA4978091.4434948390628044No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT4959671.438153598760693No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA4877291.4142659019046713No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA4532631.3143249745145706No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA4187611.2142796580631907No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTC3938781.142126518846343No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAT3504261.0161289218825338No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT3311000.9600893941525656No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG2308080.669273068213728No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT2195410.636602191729533No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT1906490.5528241706608048No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCG1884580.5464709364035161No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1848520.5360146320987316No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTCGT1828890.530322528562877No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCGT1828770.5302877322091173No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATCA1828010.5300673553019726No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT1572720.4560410123743953No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1504540.4362708840466026No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCGTA1465500.42495047029011923No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA1410290.40894124786451874No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC1264230.36658828594669224No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1215340.35241167148576835No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT1214320.3521159024788111No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA1105610.3205933056686856No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA1065940.309090211054964No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT1028850.2983352380470755No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG1015790.29454823487956344No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT981290.28454428317365477No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT957570.27766620391382424No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA950360.2755755229920967No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA937940.2719741003779696No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTCTGTAGGCACCATC915330.26541788739041616No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA906650.26290095113513245No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCTCGT842820.24439219063112816No Hit
TACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCT786430.22804080406022414No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCTC778720.22580513833116456No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATA761730.22087855457802288No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT760950.22065237827858494No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC737860.21395697987599277No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC727460.21094129588348698No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCGT724990.21022507093526682No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT707850.20525499173992554No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT669260.19406506431004109No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT652960.18933855959101759No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT644130.1867781278935343No Hit
AGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATATCTCG589230.17085879604847967No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATATC586800.1701541698848461No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCTC578460.1677358232985482No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATATCT577800.16754444335286994No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA577800.16754444335286994No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA543100.15748249772402848No Hit
TTCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCA537450.1558441694011768No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTCTGTAGGCACCATCAATATCTCGT535500.15527872865258194No Hit
AGGATAGCTGGCGCTCTCGCTCTTTTTCTGTAGGCACCATCAATATCTCG535380.15524393229882227No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT530390.1537969839216488No Hit
GACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC529720.15360270427982392No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT506930.14699429676163095No Hit
CTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA488870.14175744552079877No Hit
GGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT485490.1407773482232344No Hit
TGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCCTGTAGGCACCATCA468120.1357405760165204No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT461320.13376878263680506No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCT458790.13303515951170508No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCTGTAGGCACCATCAATA451580.13094447858997751No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATATCTCG447380.12972660620838863No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATAT442690.1283666487156144No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC441960.12815497089690966No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT434050.12586131124491726No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT432680.12546405287282755No Hit
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATAT425310.12332697681275374No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTC423890.12291521995993082No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC419430.12162195547852932No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTC418160.12125369406790602No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCTGTAGGCACCATCAATATCTCGT414350.12014890983603611No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA398340.11550649630526516No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATATCTC388490.11265029560082458No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAAT379070.10991878183068952No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA374070.10846893375736942No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT369010.1070016875071695No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATATCTCG366370.10623616772445649No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATATC363210.1053198637421182No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT360190.10444415550583286No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATC347130.10065715233832079No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATA346580.10049766905025556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)