FastQCFastQC Report
Mon 25 Sept 2023
SRR2724733.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724733.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47279599
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTAT9778762.0682831933494192No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT9316161.9704397239071338No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9256381.957795792641981Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT7114971.5048710544266672No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT7004871.481584054890144No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA6129571.2964513510362048No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC5324781.1262320562405785No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCG5011791.0600322561957431No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG4792551.0136613045300997No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCGT4634820.9803001924783669No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATT4408910.9325184843467051No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC4395450.9296715904887433No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC4270650.9032754275263628No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT3087270.6529814265133678No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTA3087070.6529391249701589No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC3083390.6521607765751144No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA3014670.6376259663285215No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG2821600.5967901715917684No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT2820480.5965532829497983No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTA2639290.5582301998796563No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC2534180.5359986238461963No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATTCGTAT2088250.4416809880303765No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2044190.43236195806144634No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA1921020.4063105526762188No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTATG1859830.3933683955314427No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1846060.3904559342815069No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGTATGC1737240.367439664621521No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTC1703550.3603139696679746No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTC1638680.34659346412815384No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATT1614780.3415384297146852No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATTCG1563770.33074942111924427No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1553670.3286131931871926No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1402440.29662688128975034No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1350270.2855925237436976No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCG1338030.28300366929931026No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATT1282490.2712565307501868No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1269800.26857249783357934No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATTCGTA1251260.2646511447781103No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATTCGTA1245970.2635322689602338No Hit
GGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCGT1079610.22834584531903498No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1078640.2281406828344716No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA1078630.22813856775731112No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATTCGT1065850.2254354991462597No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1065380.22533609051971865No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC1060410.22428489717097644No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA1011990.2140436935600913No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT991270.20966125368364485No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATTCG983400.20799668795837292No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC971220.2054205239769483No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA970530.2052745836528774No Hit
GGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCGTA969250.2050038537763402No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATTCGT967700.2046760168164709No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATTCGTA930620.19683331070553287No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG918180.1942021547179366No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA895710.18944957633841183No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATTCGT876700.1854288146564018No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATTCG856940.18124942218735823No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT853140.1804456928663883No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT822640.1739947075270245No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA812730.17189866606102136No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG803470.1699401046104473No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATTCGTA801960.16962072795921979No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGT799500.16910041897774977No Hit
GACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTAT786810.16641638606114234No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATTC779590.1648893003512995No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCGT773180.1635335358914529No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT739230.15635284893173482No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT736580.1557923534842163Illumina Single End Adapter 1 (95% over 22bp)
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTCGTA727900.15395646650894818No Hit
CCCTACCTACTATCCAGCGAAACCACAGCCCTGTAGGCACCATCAATTCG726340.15362651447191844No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATTCGTAT697480.14752240178686793No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATTCGT672520.14224316919439187No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC669550.14161499127773905No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATTC662730.14017250865431408No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATTCGT658350.13924610485803823No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATTCGT652410.13798974902473263No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATTCGT636570.13463946680258435No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT625160.13222616376251414No Hit
GGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATTCGTA621140.1313759027440144No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA598590.12660640374720608No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATTC588220.12441306873182235No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCA580210.1227188919263042No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATT578390.12233394788310281No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT576380.12190881737385294No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATTCGTATG566590.11983815683377518No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGTA565670.11964356973501403No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG552860.11693415589248124No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA550680.11647306907150376No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATTCGT537550.11369597275983664No Hit
GAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAATTC532940.1127209221888705No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA532650.11265958495121754No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT531490.11241423600060567No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATTC514270.10877207313031569No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCTGTAGGCACCAT513870.1086874700438978No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT510690.1080148755068756No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATTCGT500280.10581308018285011No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATCA498070.1053456481303913No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC489700.10357532854709704No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCG483170.10219418316132504No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT481500.10184096527553037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)