FastQCFastQC Report
Mon 25 Sept 2023
SRR2724734.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724734.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79998985
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA16683292.085437708990933No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT15024441.8780788281251317No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA11109591.388716369338936No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA10912891.3641285573810717No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA10522581.3153391883659524No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA10001461.2501983618917165No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG8902291.1128003686546772No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT8629781.0787361864653657No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8525551.0657072711610027No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC8477471.0596971949081604No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6167190.7709085309019859No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTC6025100.7531470555532673No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG5790960.7238791842171498No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT5295020.6618858976773269No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA5168950.6461269477356494No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT4820530.6025738951562948No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAT4277810.5347330344253742No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA4059540.5074489382584041No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG4022220.5027838790704657No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT3554190.44427938679471995No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT3516690.43959182732130914No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA3431010.42888169143646016No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3309180.413652748219243No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCG3291580.4114527203063889No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCGT3102750.3878486708300112No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT3050200.38127983748793814No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT2973200.37165471536920125No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCTCGT2914590.364328372416225No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT2791180.3489019266931949No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC2713510.3391930535118664No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2679990.33500300035056696No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT2454310.30679264243165083No Hit
CCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCGTA2402730.30034506062795674No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2264930.2831198420829964No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC2207580.2759510011283268No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATATCTC2190410.2738047238974345No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2103400.2629283359032618No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT2064310.2580420239081783No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA2062630.2578320212437695No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG1879220.2349054803632821No Hit
TACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCT1876150.2345217254943922No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT1858790.23235169796216787No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1831210.2289041542214567No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT1819220.2274053852058248No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA1815450.22693412922676456No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1778420.22230532049875382No Hit
ACCCGTCGCCGCGCTCTCTCTCTCTCCCCTGTAGGCACCATCAATATCTC1720720.21509272898899903No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAAT1717470.21468647383463677No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCGT1690640.21133268128339378No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT1680300.21004016488459198No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC1656220.20703012669473245No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT1584170.19802376242648578No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA1576560.19707250035734827No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1562040.19525747732924362No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCCTGTAGGCACCATCAA1511310.18891614687361347No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATCA1495680.18696237208509583No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATAT1436620.1795797784184387No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA1348320.16854213837838067No Hit
TTCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCA1345350.16817088366808652No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT1333450.16668336479519083No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC1310130.1637683278106591No Hit
GCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCTC1298350.1622958091280783No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC1278490.15981327763095995No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC1273360.15917201949499735No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT1253780.1567244884419471No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATATCT1241060.15513446826856617No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTC1239640.15495696601650635No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT1225240.1531569431787166No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT1205000.1506269110789343No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT1191260.1489093892878766No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG1183630.14795562718701993No Hit
CACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC1181820.14772937431643665No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA1155340.14441933232027881No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT1141690.14271306067195727No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT1120360.14004677684348119No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCTGTAGGCACCATC1096160.13702173846330676No Hit
CTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1018700.12733911561502936No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA1016820.12710411263342902No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC1003040.12538159077893302No Hit
TGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCCTGTAGGCACCATCA996500.12456408040677017No Hit
GGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT994680.12433657752032728No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCTCGT993160.12414657510967171No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAT983810.12297781028096794No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATCT978540.12231905192297127No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCCTGTAGGCACCATC977500.12218905027357534No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATATC962920.12036652715031324No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATA957080.1196365178883207No Hit
AGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATATCTCG936970.11712273599471293No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT933920.1167414811575422No Hit
GGAGGTGGAGCACGAGCGTACGCGTTAGGACCTGTAGGCACCATCAATAT927770.11597272140390281No Hit
AGGATAGCTGGCGCTCTCGCTCTTTTTCTGTAGGCACCATCAATATCTCG924380.11554896602750647No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT920280.11503645952508022No Hit
TCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATATC897020.11212892263570594No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTCTGTAGGCACCATCAATATCTCGT886320.1107914056659594No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATATC884490.11056265276365694No Hit
TGAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAATA873060.10913388463616132No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCTGTAGGCACCAT851440.10643135034775753No Hit
GACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC850020.10625384809569771No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAAT830440.10380631704264749No Hit
AGTACGAATACAGACCGTGAAAGCGGGGCCTCCTGTAGGCACCATCAATA821470.10268505281660761No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCTGTAGGCACCATCAATATCTCGT818110.10226504748779No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCCTGTAGGCACCATCA811630.10145503721078462No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTCTGTAGGCACCATC811340.1014187867508569No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATATCTC805910.100740028139107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)