FastQCFastQC Report
Mon 25 Sept 2023
SRR2724736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58933265
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTC42287587.175502663902976No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT41483657.039089044192613No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG35736636.063914836552837No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA34364025.831005629842501No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA34255435.812579703500222No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA10898521.8492985243563207No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA9352831.5870205053122377No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC8917651.5131776595102953No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA8818321.4963230019582319No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8483571.4395214655084867No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGT5863750.9949813573030444No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4838970.821093146629497No Hit
GGGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCT4076010.6916314580568377No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCT4028150.6835104079164798No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCT3753450.6368983629194819No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC3645810.6186336358591367No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTC3606510.6119650760907274No Hit
TGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG3218920.546197465896383No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3004140.5097528535030258No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATATCTCG2930850.4973167531104886No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2750190.4666617401903662No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT2633550.44686986203801876No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC2505360.4251181399842686No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT2358890.4002646043792075No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2284770.3876876667192968No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2156520.3659257636582667No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2042390.3465597909771332No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC1731540.2938136890939268No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA1683460.2856553085935422No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1664590.2824533818039778No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1627320.2761292794485423No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT1522750.25838548059402444No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT1474260.2501575298772264No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA1444910.24517732048275281No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1404150.2382610228705299No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGTA1376390.2335506101689767No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1267230.21502796425753776No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1131210.19194762075374577No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC1128150.19142838938246506No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT1059170.1797236246795422No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1057550.1794487374829818No Hit
AGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1038360.17619251198792396No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1007400.1709391122314367No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC1001370.16991592099979527No Hit
AGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATATCTCG986370.16737066917979176No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA978360.16601150470790987No Hit
TGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTC968030.16425867462120078No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA961270.16311161446765252No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC946780.1606529012095291No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC935490.1587371750063398No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGCTCGCACCTGTAGGCACCATC834140.1415397568758493No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG824000.13981916664552696No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTCTGTAGGCACCATCAATATCTCGT818070.13881294375935221No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA803420.1363270811484821No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA802720.13620830273021528No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA786020.13337458903727803No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATATCT770010.13065795692806093No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT761400.1291969823833789No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATATCTC747840.1268960747380957No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT739910.1255504849425872No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT661860.11230669130583551No Hit
TAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC655500.11122750453415402No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC652520.11072184783924663No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT642980.1091030676817244No Hit
TGAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAATA641930.10892490005432415No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCT629670.10684458090010794No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGTAGGCACCATCAATATCT623230.10575181945205309No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTCG598760.101599665316354No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA594370.1008547549503663Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)