FastQCFastQC Report
Mon 25 Sept 2023
SRR2724737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2724737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44514301
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA16167323.63193841907121No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA13610613.0575814275955944No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT8850761.9882958512591267No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC6469631.4533823635689573No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3436990.772109170039534No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAACCTGTAGGCACCATCAATATCT2742510.6160963866421265No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2591510.5821747038103553Illumina PCR Primer Index 6 (96% over 29bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2397640.5386224081110472No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCACCATCA2385540.5359041805463821No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA2313570.519736342709279No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA2306480.518143596144529No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA2239150.5030181199520576No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT2214850.4975592001321104No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA1989010.446824942842526No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATC1547460.3476321014228663No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAA1537900.34548447699987467No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT1502620.33755893415017346No Hit
CGGAATGCCTAAAGGAAAGATCCAAAAAGATCTGTAGGCACCATCAATAT1493470.33550341495871183No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATATC1388300.31187729983674234No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGCTGTAGGCACCATCAATATCT1384890.31111125388670036No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1293750.29063693485830544No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAAT1259620.28296973595070046No Hit
AAAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATA1205920.2709061970893354No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCCTGTAGGCACCATCAATAT1137960.2556391933459766No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCTGTAGGCACCAT1130970.25406891147184363No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA1102280.24762379173380705No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGACTGTAGGCACCATCAAT1083860.24348579572214332No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1055010.23700473247911946No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCTGTAGGCACCATCAATATC1020000.2291398442940843No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCAC1000300.22471430024252206No Hit
GGAATGCCTAAAGGAAAGATCCAAAAAGATCTGTAGGCACCATCAATATC985870.22147264538647926No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCCTGTAGGCACCATCAATATC985690.22143220894336857No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA980310.22022360858817033No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCCTGTAGGCACCATCAAT977820.2196642377918054No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAACTGTAGGCACCATCAATATCT946210.21256314908775048No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA897890.20170821058158364No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATGCTGTAGGCACCATCAAT871700.1958247081089738No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCCTGTAGGCACCATCAAT867490.19487894463399527No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCATC854380.19193382369409778No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAT847660.19042419648463085No Hit
TGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACC845670.18997714914135122No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCCTGTAGGCACCATCA833400.1872207316026371No Hit
AAAAAGTAAGTTTAGATTATAGCCAAAAGAGGCTGTAGGCACCATCAATA831200.1867265084090616No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATA820950.18442387762081225No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCCTGTAGGCACCATCAATAT807040.1812990391559782No Hit
AAAAAGTAAGTTTAGATTATAGCCAAAAGAGCTGTAGGCACCATCAATAT799750.179661363209994No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCTGTAGGCACCATCAATATC791250.17775186450754332No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT772170.17346560153780693No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT766990.17230193056384285No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGCTGTAGGCACCATC716370.16093030417348347No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCAA713020.1601777370378117No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT709380.15936002229935048No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAT706110.1586254269161724No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT684990.15388088425784785No Hit
AAATAGAGCCACCTTACAAATAAGCGCTCTCCTGTAGGCACCATCAATAT681000.15298454310222687No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTGTAGGCACCATCAATATC675250.1516928233917455No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA673360.15126824073908293No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT672410.1510548261782208No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCCTGTAGGCACCATCAATATCT657990.14781541779123972No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT648270.1456318498632608No Hit
AACCTAGACTTACAAGTCAAAGTAAAATCCTGTAGGCACCATCAATATCT630810.1417095148815209No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCTGTAGGCACCATCAA628150.1411119541111069No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCACCATCAATAT623100.13997748723494502No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCT620100.139303546516433No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAT605530.13603044109352633No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATA595270.13372556383621526No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATATCT534450.12006253900291504No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT528530.11873262931838466No Hit
AATAATCCACCTATAACTTCTCTGTTAACCCAACCTGTAGGCACCATCAA498570.11200220800951137No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT497350.11172813878398316No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA495730.11136421079598667No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTAGGCA494650.11112159213732234No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT488060.10964116902565761No Hit
GGAAAAAACCTTTAATAGTGAATAATTAACCTGTAGGCACCATCAATATC487540.10952435263444887No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCTGTAGGCACCA486070.10919412168237798No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACTGTAGGCACCATCAATATCT482320.10835169578423798No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCAC476920.10713860249091633No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAT472570.10616138844907393No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCCCTGTAGGCACCATCAATAT460620.10347685792033441No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCTGTAGGCACCATCAA459260.1031713381279423No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAAT452190.10158308450131566No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC450400.10118096653927014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)